Huber F, Wiedemann M, Heinrich G, Salama Z, Jaeger H
Analytical Research Centre of the LAB GmbH, Neu Ulm, Fed. Rep. of Germany.
Arzneimittelforschung. 1990 Aug;40(8):926-31.
An improved high performance liquid chromatographic assay (HPLC) is presented for the quantitative determination of prednisone and its main metabolite prednisolone. This HPLC assay, employing a UV-detector and dexamethasone as an internal standard, provides a selective and sensitive determination of glucocorticoids in plasma. The advantage of this method is the possibility of simultaneously measuring exogenic and endogenic glucocorticoids. The lower limit of quantification is 5 ng/ml.
