Department of Gene Technology, Tallinn University of Technology, Akadeemia tee 15, 12618 Tallinn, Estonia.
Virus Res. 2012 Jun;166(1-2):125-9. doi: 10.1016/j.virusres.2012.03.005. Epub 2012 Mar 15.
Cocksfoot mottle virus (CfMV) localization in oat plants was analyzed during three weeks post infection by immunohistochemical staining to follow its spread through different tissues. In early stages of infection, the virus was first detectable in phloem parenchyma and bundle sheath cells of inoculated leaves. Bundle sheath and phloem parenchyma were also the cell types where the virus was first detected in stems and systemic leaves of infected plants. In later stages of infection, CfMV spread also into the mesophyll surrounding vascular bundles and was seldom detected in xylem parenchyma of inoculated leaves. In systemic leaves, CfMV was not detected from xylem. Moreover, sometimes it was found from phloem only. In straw and roots, CfMV was detected both from phloem and xylem. According to our observations, CfMV predominantly moves through phloem, which makes the systemic movement of CfMV different from that of another monocot-infecting sobemovirus, Rice yellow mottle virus (RYMV).
雀麦花叶病毒（CfMV）在感染后三周内的定位通过免疫组织化学染色进行了分析，以跟踪其在不同组织中的传播。在感染的早期阶段，病毒首先可在接种叶片的韧皮部薄壁组织和维管束鞘细胞中检测到。在感染植物的茎和系统叶片中，病毒也是首先在维管束鞘和韧皮部薄壁组织中检测到的细胞类型。在感染的后期阶段，CfMV 也传播到围绕维管束的叶肉中，并且在接种叶片的木质部薄壁组织中很少检测到。在系统叶片中，从木质部未检测到 CfMV。此外，有时仅从韧皮部检测到 CfMV。在秸秆和根中，CfMV 既存在于韧皮部也存在于木质部中。根据我们的观察，CfMV 主要通过韧皮部移动，这使得 CfMV 的系统运动与另一种感染单子叶植物的 sobemovirus，水稻黄花叶病毒（RYMV）的系统运动不同。
