The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, School of Life Sciences, Shandong University, Jinan 250100, PR China.
Gene. 2012 May 10;499(1):154-9. doi: 10.1016/j.gene.2012.03.011. Epub 2012 Mar 8.
The St genome, which is present in nearly half of all Triticeae species, originates from the genus Pseudoroegneria. However, very little is known about the high molecular weight (HMW) subunits of glutenin which are encoded by the St genome. In this paper, we report the isolation from Pd. libanotica of four sequences encoding HMW subunits of glutenin. The four genes were all small compared to standard glutenin genes. All four sequences resemble y-type glutenins rather than x-types. However, their N-terminal domains contain a glutamine residue which is present in all x-type, but very few y-type subunits, and their central repetitive domains included some irregular motifs. The indication is therefore that the Glu-1St genes evolved earlier than other modern day homoeologues, so that they represent an intermediate state in the divergence between x- and y-type subunits. No x-type Glu-1St subunit genes were identified.
St 基因组存在于近一半的小麦族物种中，它源自假鹅观草属。然而，人们对 St 基因组编码的高分子量（HMW）麦谷蛋白亚基知之甚少。在本文中，我们报道了从小冰麦中分离到四个编码 HMW 麦谷蛋白亚基的序列。与标准麦谷蛋白基因相比，这四个基因都较小。这四个序列都类似于 y 型麦谷蛋白，而不是 x 型。然而，它们的 N 端结构域含有一个谷氨酰胺残基，该残基存在于所有的 x 型，但很少存在于 y 型亚基中，而且它们的中心重复结构域包含一些不规则的基序。因此，表明 Glu-1St 基因比其他现代同源基因更早进化，因此它们代表了 x 型和 y 型亚基之间分化的中间状态。没有鉴定到 x 型 Glu-1St 亚基基因。
