National University of Ireland, Galway, Ireland.
J Environ Sci Health B. 2012;47(4):315-25. doi: 10.1080/03601234.2012.640915.
Wilt is a serious disease of the guava crop in India. Fusarium oxysporum f. sp. psidii and F. solani have been reported as causative agents of this disease. In this study, 42 isolates each of F. oxysporum f. sp. psidii and F. solani, were isolated from guava cultivars and characterized by randomly amplified polymorphic DNA (RAPD) method. Thirty RAPD primers were tested in the genome of Fusarium spp. and the number of scorable bands for corresponding primer ranged from 1-8 with an average of 5 bands per individual. DNA band size ranged from 200 bp to 5090 bp. A 0.21 per cent polymorphism was found in individual isolates of F. solani indicating that the 42 isolates were similar. However, a 2.58 percent polymorphism among individual isolates of F. oxysporum f.sp. psidii showed a higher level of genetic diversity. Cluster analysis of the RAPD band patterns clearly separated the isolates of F. oxysporum f.sp. psidii into three clusters. Two clusters were formed with F. solani isolates, showing a higher degree of similarity. Unique fingerprint profiles generated by the PCR-RAPD can be exploited for genetic characterization purposes.
枯萎病是印度番石榴作物的一种严重病害。尖孢镰刀菌古巴专化型和茄类镰刀菌已被报道为该病的病原体。在这项研究中，从番石榴品种中分离出了 42 株尖孢镰刀菌古巴专化型和茄类镰刀菌的分离株，并通过随机扩增多态性 DNA (RAPD) 方法进行了特征描述。在 Fusarium spp. 的基因组中测试了 30 个 RAPD 引物，对应引物的可评分带数量从 1-8 不等，平均每个个体有 5 个带。DNA 带大小从 200bp 到 5090bp 不等。在茄类镰刀菌的单个分离株中发现了 0.21%的多态性，表明这 42 个分离株是相似的。然而，尖孢镰刀菌古巴专化型的单个分离株之间存在 2.58%的多态性，表明其遗传多样性水平较高。RAPD 带型的聚类分析清楚地将尖孢镰刀菌古巴专化型的分离株分为三个聚类。茄类镰刀菌的两个聚类形成，显示出更高的相似性。PCR-RAPD 产生的独特指纹图谱可用于遗传特征描述目的。
