Department of Medical Oncology, University Hospital Virgen del Rocío, Instituto de Biomedicina de Sevilla, Seville, Spain.
Dis Markers. 2012;32(4):221-30. doi: 10.3233/DMA-2011-0882.
The identification of new less invasive biomarkers is necessary to improve the detection and prognostic outcome of respiratory pathological processes. The measurement of miRNA expression through less invasive techniques such as plasma and serum have been suggested to analysis of several lung malignancies including lung cancer. These studies are assuming a common deregulated miRNA expression both in blood and lung tissue. The present study aimed to obtain miRNA representative signatures both in plasma and bronchoalveolar cell fraction that could serve as biomarker in respiratory diseases. Ten patients were evaluated to assess the expression levels of 381 miRNAs. We found that around 50% miRNAs were no detected in both plasma and bronchoalveolar cell fraction and only 20% of miRNAs showed similar expression in both samples. These results show a lack of association of miRNA signatures between plasma and bronchoalveolar cytology in the same patient. The profiles are not comparable; however, there is a similarity in the relative expression in a very small subset of miRNAs (miR-17, miR-19b, miR-195 and miR-20b) between both biological samples in all patients. This finding supports that the miRNAs profiles obtained from different biological samples have to be carefully validated to link with respiratory diseases.
为了改善呼吸病理过程的检测和预后结果，有必要鉴定新的微创生物标志物。通过血浆和血清等微创技术测量 miRNA 表达，已被提议用于分析包括肺癌在内的几种肺癌。这些研究假设血液和肺组织中存在共同的 miRNA 表达失调。本研究旨在获得既能代表血浆又能代表肺泡细胞成分的 miRNA 特征，以作为呼吸疾病的生物标志物。对 10 名患者进行评估以检测 381 种 miRNA 的表达水平。我们发现，大约 50%的 miRNA 在血浆和肺泡细胞成分中均未检测到，只有 20%的 miRNA 在两种样本中的表达相似。这些结果表明，同一患者血浆和肺泡细胞学之间的 miRNA 特征之间缺乏关联。这些图谱不可比较；然而，在所有患者的两种生物样本中，非常小的 miRNA 亚群（miR-17、miR-19b、miR-195 和 miR-20b）的相对表达存在相似性。这一发现支持从不同生物样本获得的 miRNAs 图谱必须经过仔细验证才能与呼吸疾病相关联。
