Braley-Mullen H
Eur J Immunol. 1977 Nov;7(11):775-81. doi: 10.1002/eji.1830071106.
Mice primed with a thymus-dependent form of Type 3 pneumococcal polysaccharide (S3), i.e. S3 coupled to erythrocytes (S3-RBC) produces S3-specific IgG antibody after secondary challenge with S3-RBC. When mice are depleted of T cells by treatment with anti-lymphocyte serum (ALS) at the time of priming, no IgG antibody is produced after secondary challenge. In order to determine the cellular basis for this phenomenon, various combinations of T and/or B cells from ALS-treated or normal primed mice were transferred to irradiated recipients prior to secondary challenge with S3-RBC. The results indicated that T cells were required at the time of priming with S3-RBC in order to (a) prevent the induction of tolerance in S3-specific B cells in mice primed with high doses of S3-RBC, and (b) induced differentiation of IgG-producing B cell precursors to Bgamma memory cells in mice primed with low doses of antigen.
用胸腺依赖型3型肺炎球菌多糖（S3）致敏的小鼠，即与红细胞偶联的S3（S3-RBC），在用S3-RBC再次攻击后会产生S3特异性IgG抗体。当在致敏时用抗淋巴细胞血清（ALS）处理使小鼠的T细胞耗竭时，再次攻击后不会产生IgG抗体。为了确定这种现象的细胞基础，在对S3-RBC进行再次攻击之前，将来自ALS处理或正常致敏小鼠的各种T细胞和/或B细胞组合转移到经辐射的受体中。结果表明，在用S3-RBC致敏时需要T细胞，以便（a）防止在用高剂量S3-RBC致敏的小鼠中诱导S3特异性B细胞的耐受性，以及（b）在用低剂量抗原致敏的小鼠中诱导产生IgG的B细胞前体分化为Bγ记忆细胞。
