Department of Anesthesiology and Intensive Care Medicine, Oita University Faculty of Medicine, Yufu City, Oita, Japan.
J Surg Res. 2012 Jul;176(1):220-5. doi: 10.1016/j.jss.2011.07.039. Epub 2011 Aug 22.
Acute kidney injury (AKI), which occurs during renal transplantation and cardiovascular surgery, is a major clinical problem associated with high mortality, and has limited treatment options. Anti-inflammation therapy has been suggested to improve the course and outcome of AKI. In this study, we hypothesized that ESeroS-GS, a vitamin E derivative, inhibits cytokine production and prevents renal ischemia-reperfusion (I/R) injury in rats.
Rats received an intravenous infusion of ESeroS-GS or saline, and underwent experimentally-induced renal I/R injury or sham treatment. Rats were sacrificed after 60 min of ischemia and 24 h of reperfusion. To evaluate the renal protective effects of ESero-GS, renal function was examined, kidneys were histologically assessed, levels of myeloperoxidase (MPO) and serum cytokines were measured, and caspase 3/7 activity was determined.
ESeroS-GS attenuated I/R-induced histologic alterations, reduced levels of MPO and serum BUN, Cre, TNF-α, and IL-6, and decreased caspase 3/7 activity in kidneys of rats subjected to renal I/R injury.
ESeroS-GS attenuated renal injury after I/R by reducing serum cytokine levels. Our findings suggest that ESeroS-GS may have therapeutic potential against various human I/R conditions.
急性肾损伤（AKI）发生于肾移植和心血管手术期间，是一种与高死亡率相关的主要临床问题，且其治疗选择有限。抗炎治疗被认为可以改善 AKI 的病程和结局。在本研究中，我们假设 ESeroS-GS，一种维生素 E 衍生物，可抑制细胞因子的产生并预防大鼠肾缺血再灌注（I/R）损伤。
大鼠接受 ESeroS-GS 或生理盐水静脉输注，并接受实验诱导的肾 I/R 损伤或假手术处理。缺血 60 分钟和再灌注 24 小时后处死大鼠。为评估 ESero-GS 的肾保护作用，检查了肾功能，对肾脏进行了组织学评估，测量了髓过氧化物酶（MPO）和血清细胞因子的水平，并测定了 caspase 3/7 活性。
ESeroS-GS 减轻了 I/R 诱导的组织学改变，降低了大鼠肾 I/R 损伤模型中 MPO 和血清 BUN、Cre、TNF-α和 IL-6 的水平，并降低了 caspase 3/7 活性。
ESeroS-GS 通过降低血清细胞因子水平减轻了 I/R 后的肾损伤。我们的研究结果表明，ESeroS-GS 可能对各种人类 I/R 情况具有治疗潜力。
