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本文引用的文献

1
XDS.XDS.（这个词如果没有更多背景信息，很难准确翻译出更有意义的内容，直接保留原文是一种处理方式，或者音译为“克斯达斯”之类，但感觉都不太符合常规翻译场景，你可以补充更多关于这个词的信息以便我更准确翻译 ）
Acta Crystallogr D Biol Crystallogr. 2010 Feb;66(Pt 2):125-32. doi: 10.1107/S0907444909047337. Epub 2010 Jan 22.
2
The utrophin A 5'-UTR drives cap-independent translation exclusively in skeletal muscles of transgenic mice and interacts with eEF1A2.肌联蛋白 A 5'-UTR 在转基因小鼠的骨骼肌中特异性驱动无帽依赖翻译，并与 eEF1A2 相互作用。
Hum Mol Genet. 2010 Apr 1;19(7):1211-20. doi: 10.1093/hmg/ddp591. Epub 2010 Jan 6.
3
Interaction of elongation factor 1-alpha with leucine-rich repeat kinase 2 impairs kinase activity and microtubule bundling in vitro.延伸因子1-α与富含亮氨酸重复序列激酶2的相互作用在体外损害激酶活性和微管成束。
Neuroscience. 2009 Oct 6;163(2):533-9. doi: 10.1016/j.neuroscience.2009.06.051. Epub 2009 Jun 25.
4
Fine mapping of interactions between eEF1alpha protein and 3'UTR of metallothionein-1 mRNA.真核延伸因子1α（eEF1α）蛋白与金属硫蛋白-1（metallothionein-1）mRNA的3'非翻译区（3'UTR）之间相互作用的精细定位
Biochem Biophys Res Commun. 2009 Aug 14;386(1):82-8. doi: 10.1016/j.bbrc.2009.05.146. Epub 2009 Jun 6.
5
Translation elongation factor 1A is a component of the tombusvirus replicase complex and affects the stability of the p33 replication co-factor.翻译延伸因子1A是番茄丛矮病毒复制酶复合体的一个组成部分，并影响p33复制辅助因子的稳定性。
Virology. 2009 Mar 1;385(1):245-60. doi: 10.1016/j.virol.2008.11.041. Epub 2009 Jan 7.
6
Coordination of eukaryotic translation elongation factor 1A (eEF1A) function in actin organization and translation elongation by the guanine nucleotide exchange factor eEF1Balpha.鸟嘌呤核苷酸交换因子eEF1Bα对真核生物翻译延伸因子1A（eEF1A）在肌动蛋白组织和翻译延伸过程中的功能进行协调。
J Biol Chem. 2009 Feb 13;284(7):4739-47. doi: 10.1074/jbc.M807945200. Epub 2008 Dec 18.
7
Interaction between the cellular protein eEF1A and the 3'-terminal stem-loop of West Nile virus genomic RNA facilitates viral minus-strand RNA synthesis.细胞蛋白eEF1A与西尼罗河病毒基因组RNA的3'-末端茎环之间的相互作用促进了病毒负链RNA的合成。
J Virol. 2007 Sep;81(18):10172-87. doi: 10.1128/JVI.00531-07. Epub 2007 Jul 11.
8
Mouse translation elongation factor eEF1A-2 interacts with Prdx-I to protect cells against apoptotic death induced by oxidative stress.小鼠翻译延伸因子eEF1A-2与Prdx-I相互作用，保护细胞免受氧化应激诱导的凋亡死亡。
J Cell Biochem. 2007 Feb 1;100(2):267-78. doi: 10.1002/jcb.20969.
9
The integration of macromolecular diffraction data.大分子衍射数据的整合
Acta Crystallogr D Biol Crystallogr. 2006 Jan;62(Pt 1):48-57. doi: 10.1107/S0907444905039107. Epub 2005 Dec 14.
10
A critical role for eukaryotic elongation factor 1A-1 in lipotoxic cell death.真核延伸因子1A-1在脂毒性细胞死亡中的关键作用。
Mol Biol Cell. 2006 Feb;17(2):770-8. doi: 10.1091/mbc.e05-08-0742. Epub 2005 Nov 30.

哺乳动物翻译延伸因子eEF1A2的纯化、结晶及初步X射线晶体学分析。

Purification, crystallization and preliminary X-ray crystallographic analysis of mammalian translation elongation factor eEF1A2.

作者信息

Yaremchuk A, Shalak V F, Novosylna O V, Negrutskii B S, Crépin T, El'skaya A V, Tukalo M

机构信息

State Key Laboratory of Molecular and Cellular Biology, Institute of Molecular Biology and Genetics, 150 Zabolotnogo Street, 03680 Kyiv-143, Ukraine.

出版信息

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Mar 1;68(Pt 3):295-7. doi: 10.1107/S1744309112000243. Epub 2012 Feb 22.

DOI:10.1107/S1744309112000243
PMID:22442226
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3310534/
Abstract

Translation elongation factor eEF1A2 was purified to homogeneity from rabbit muscle by two consecutive ion-exchange column-chromatography steps and this mammalian eEF1A2 was successfully crystallized for the first time. Protein crystals obtained using ammonium sulfate as precipitant diffracted to 2.5 Å resolution and belonged to space group P6(1)22 or P6(3)22 (unit-cell parameters a = b = 135.4, c = 304.6 Å). A complete native data set was collected to 2.7 Å resolution.

摘要

通过连续两步离子交换柱色谱法从兔肌肉中纯化得到了均一的翻译延伸因子eEF1A2，并且首次成功地使这种哺乳动物的eEF1A2结晶。以硫酸铵作为沉淀剂获得的蛋白质晶体衍射分辨率达到2.5 Å，属于空间群P6(1)22或P6(3)22（晶胞参数a = b = 135.4，c = 304.6 Å）。收集了一个完整的天然数据集，分辨率达到2.7 Å。