Saikhun K, Faisaikarm T, Ming Z, Lu K H, Kitiyanant Y
1Institute of Science and Technology for Research and Development, Mahidol University, Salaya, Nakhon Pathom 73170, Thailand.
Animal. 2008 Oct;2(10):1486-90. doi: 10.1017/S1751731108002541.
This study was conducted to investigate the effects of capacitating agents added at in vitro fertilization (IVF) and antioxidants supplemented during in vitro culture (IVC) on the development of buffalo embryos. In experiment I, in vitro embryo development of buffalo embryos was compared when the IVF medium was supplemented with heparin, caffeine and calcium ionophore A23187 either alone or in combination. There was no significant difference (P > 0.05) in the cleavage rates of oocytes among the treatment groups but the development rate to the blastocyst stage and the cell numbers of blastocyst in the heparin-treated group were significantly higher (P < 0.05) than that of other treatments. In experiment II, in vitro embryo development of buffalo embryos was compared when IVC medium was supplemented with either α-tocopherol (250 and 500 μM) or l-ascorbic acid (250 and 500 μM). The rate of development to the blastocyst stage of embryos cultured in medium supplemented with 250 μM α-tocopherol (33%, 41/123) and 250 μM l-ascorbic acid (31%, 38/123) was significantly higher (P < 0.05) than that of those cultured in medium alone (19%, 20/108) but not significantly different (P < 0.05) from medium supplemented with either 500 μM α-tocopherol (24%, 30/123) or 500 μM l-ascorbic acid (25%, 33/133). These results suggest that buffalo spermatozoa treated with heparin were suitable for IVF and that α-tocopherol and l-ascorbic acid added during IVC increased the rate of buffalo embryo development.
本研究旨在探讨体外受精（IVF）时添加获能剂以及体外培养（IVC）期间补充抗氧化剂对水牛胚胎发育的影响。在实验I中，比较了在IVF培养基中单独或联合添加肝素、咖啡因和钙离子载体A23187时水牛胚胎的体外发育情况。各处理组卵母细胞的裂解率无显著差异（P>0.05），但肝素处理组的囊胚发育率和囊胚细胞数均显著高于其他处理组（P<0.05）。在实验II中，比较了在IVC培养基中添加α-生育酚（250和500μM）或L-抗坏血酸（250和500μM）时水牛胚胎的体外发育情况。在添加250μMα-生育酚（33%，41/123）和250μM L-抗坏血酸（31%，38/123）的培养基中培养的胚胎，其囊胚发育率显著高于单独在培养基中培养的胚胎（19%，20/108）（P<0.05），但与添加500μMα-生育酚（24%，30/123）或500μM L-抗坏血酸（25%，33/133）的培养基培养的胚胎无显著差异（P<0.05）。这些结果表明，用肝素处理的水牛精子适用于IVF，并且在IVC期间添加α-生育酚和L-抗坏血酸可提高水牛胚胎的发育率。
