Jezierny D, Mosenthin R, Sauer N, Eklund M
Institute of Animal Nutrition, University of Hohenheim, Emil-Wolff-Str. 10, 70593 Stuttgart, Germany.
Animal. 2010 Dec;4(12):1987-96. doi: 10.1017/S1751731110001114.
The study was conducted to validate in vitro prediction of standardised ileal digestibilities (SID) of crude protein (CP) and amino acids (AA) in grain legumes for growing pigs using six different cultivars of faba beans (Vicia faba), six different cultivars of field peas (Pisum sativum), and five different cultivars of lupins (Lupinus spp.). The SID for CP and AA were predicted from in vitro analysis by means of a two-step enzymatic method using pepsin and pancreatin incubations. In vitro predicted SID values of CP and AA were generally higher than the corresponding SID values measured in vivo. There were strong linear relationships (r2 = 0.73 for Lys to r2 = 0.91 for Cys and Trp) between in vivo and in vitro predicted SID values in the assay feed ingredients if grain legume species (i.e. faba beans, field peas and lupins) was included as a covariate in multiple linear regression analysis. However, to rapidly and accurately predict SID of CP and AA in individual batches of various feed ingredients, further studies are warranted.
本研究旨在验证使用六种不同品种的蚕豆（蚕豆属）、六种不同品种的豌豆（豌豆属）和五种不同品种的羽扇豆（羽扇豆属）对生长猪的豆类粗蛋白（CP）和氨基酸（AA）标准化回肠消化率（SID）进行体外预测。CP和AA的SID通过使用胃蛋白酶和胰酶孵育的两步酶法从体外分析中预测。CP和AA的体外预测SID值通常高于体内测量的相应SID值。如果在多元线性回归分析中将豆类品种（即蚕豆、豌豆和羽扇豆）作为协变量纳入，则在测定饲料成分中，体内和体外预测的SID值之间存在很强的线性关系（从赖氨酸的r2 = 0.73到半胱氨酸和色氨酸的r2 = 0.91）。然而，为了快速准确地预测各批次不同饲料成分中CP和AA的SID，还需要进一步研究。
