Department of Laboratory Medicine, Changzheng Hospital, Second Military Medical University, 415 Fengyang Road, Shanghai, 200003, People's Republic of China.
Med Oncol. 2012 Dec;29(4):2402-8. doi: 10.1007/s12032-012-0210-3. Epub 2012 Mar 24.
Circulating microRNAs (miRNAs) have shown potential as non-invasive prognostic biomarkers in cancer. Here, we investigated whether miRNAs present in the plasma of multiple myeloma (MM) patients have prognostic utility. We evaluated global miRNA expression profiles in the plasma of 12 multiple myeloma patients and 8 healthy controls using TaqMan Low-Density Arrays. Six miRNAs (miR-148a, miR-181a, miR-20a, miR-221, miR-625, and miR-99b) that were significantly upregulated in MM were selected and further quantified independently by quantitative reverse transcription PCR in plasma from 28 MM patients and 12 healthy controls. Moreover, within the patient group, the expression levels of miR-99b and miR-221 were associated with chromosomal abnormalities t(4; 14) and del(13q), respectively. High levels of miR-20a and miR-148a were related to shorter relapse-free survival. In summary, we have identified aberrant expression of particular circulating miRNAs that are associated with the genetic subtype and survival of MM. These plasma miRNAs have potential as clinical biomarkers in MM.
循环 microRNAs（miRNAs）已显示出作为癌症非侵入性预后生物标志物的潜力。在这里，我们研究了多发性骨髓瘤（MM）患者血浆中存在的 miRNAs 是否具有预后作用。我们使用 TaqMan 低通量阵列评估了 12 名多发性骨髓瘤患者和 8 名健康对照者血浆中的全局 miRNA 表达谱。选择了在 MM 中显著上调的 6 个 miRNA（miR-148a、miR-181a、miR-20a、miR-221、miR-625 和 miR-99b），并通过定量逆转录聚合酶链反应在 28 名 MM 患者和 12 名健康对照者的血浆中进一步独立定量。此外，在患者组中，miR-99b 和 miR-221 的表达水平分别与染色体异常 t（4；14）和 del（13q）相关。高水平的 miR-20a 和 miR-148a 与较短的无病生存时间相关。总之，我们已经确定了特定循环 miRNAs 的异常表达与 MM 的遗传亚型和生存有关。这些血浆 miRNAs 在 MM 中具有作为临床生物标志物的潜力。
