运动训练对高血压患者血小板（L）-精氨酸：一氧化氮途径的上调作用。

Upregulation of platelet (L)-arginine: nitric oxide pathway after exercise training in hypertension.

机构信息

Laboratory of Membrane Transport, Department of Pharmacology and Psychobiology, State University of Rio de Janeiro, Vila Isabel, Rio de Janeiro, Brazil.

出版信息

Can J Physiol Pharmacol. 2012 Apr;90(4):501-5. doi: 10.1139/y2012-008. Epub 2012 Mar 26.

DOI:10.1139/y2012-008

PMID:22448908

Abstract

We investigated whether physical exercise can affect platelet L-arginine - nitric oxide pathway in spontaneously hypertensive rats (SHR). Sixteen male SHR and 16 Wistar Kyoto rats (WKY) were divided among exercise (EX) and sedentary (SED) groups. After 20 weeks of treadmill training, systolic blood pressure (mm Hg) was significantly lower in exercised spontaneously hypertensive rats (SHR/EX; 138 ± 8) than in sedentary spontaneously hypertensive rats (SHR/SED; 214 ± 9). Exercise significantly increased platelet L-arginine transport (pmol L-arginine·(10(9) cells)(-1)·min(-1)), assessed by incubation with L-[(3)H]-arginine, in both WKY (SED, 0.196 ± 0.054 compared with EX, 0.531 ± 0.052) and SHR (SED, 0.346 ± 0.076 compared with EX, 0.600 ± 0.049). Nitric oxide synthase (NOS) activity (pmol L-citrulline·(10(8) cells)(-1)), measured by the conversion of L-[(3)H]-arginine to L-[(3)H]-citrulline, was significantly increased in SHR/EX (0.072 ± 0.007) compared with SHR/SED (0.038 ± 0.007), but no changes were observed in WKY. The iNOS and eNOS protein levels assessed by Western blot were not affected by exercise. This upregulation of the platelet L-arginine-NO pathway may attenuate the risk of thromboembolic events, supporting the role of exercise in hypertension management.

摘要

我们研究了体育锻炼是否会影响自发性高血压大鼠（SHR）的血小板 L-精氨酸-一氧化氮途径。将 16 只雄性 SHR 和 16 只 Wistar Kyoto 大鼠（WKY）分为运动（EX）和安静（SED）组。经过 20 周的跑步机训练后，运动组 SHR 的收缩压（mmHg）明显低于安静组 SHR（SHR/EX：138±8 与 SHR/SED：214±9）。运动显著增加了 WKY（SED：0.196±0.054 与 EX：0.531±0.052）和 SHR（SED：0.346±0.076 与 EX：0.600±0.049）的血小板 L-精氨酸转运（pmol L-精氨酸·（10^9 细胞）-1·min-1），通过孵育 L-[(3)H]-精氨酸进行评估。NOS 活性（pmol L-瓜氨酸·（10^8 细胞）-1），通过 L-[(3)H]-精氨酸转化为 L-[(3)H]-瓜氨酸来测量，在 SHR/EX 中显著增加（0.072±0.007 与 SHR/SED：0.038±0.007），但在 WKY 中没有观察到变化。通过 Western blot 评估的 iNOS 和 eNOS 蛋白水平不受运动影响。血小板 L-精氨酸-NO 途径的这种上调可能会降低血栓栓塞事件的风险，支持运动在高血压管理中的作用。