Nicolini F A, Mehta J L
Division of Cardiology, University of Florida College of Medicine, Gainesville 32610.
Biochem Pharmacol. 1990 Nov 15;40(10):2265-9. doi: 10.1016/0006-2952(90)90721-v.
Previous studies have indicated a possible role for polymorphonuclear leukocytes (PMNLs) in the maintenance of hemostasis and vascular tone. We now demonstrate that unstimulated isolated PMNLs maintained at 37 degrees inhibited human platelet aggregation in a concentration- and time-dependent fashion. In addition, PMNLs increased platelet cyclic GMP concentrations. The platelet aggregation inhibitory effect of PMNLs was potentiated by superoxide dismutase and attenuated by hemoglobin and methylene blue. This inhibitory effect of PMNLs was not observed in 48-hr-old killed cells and was not modulated by aspirin treatment or by adenosine deaminase. These observations suggest that human PMNLs maintained at 37 degrees produce a substance with biological characteristics similar to those of the endothelium-derived relaxing factor.
先前的研究表明，多形核白细胞（PMNLs）在维持止血和血管张力方面可能发挥作用。我们现在证明，在37摄氏度下保存的未受刺激的分离PMNLs以浓度和时间依赖性方式抑制人血小板聚集。此外，PMNLs增加血小板环鸟苷酸浓度。超氧化物歧化酶可增强PMNLs对血小板聚集的抑制作用，而血红蛋白和亚甲蓝则可减弱该作用。在48小时龄的死亡细胞中未观察到PMNLs的这种抑制作用，阿司匹林治疗或腺苷脱氨酶也不会对其产生调节作用。这些观察结果表明，在37摄氏度下保存的人PMNLs产生一种具有与内皮衍生舒张因子相似生物学特性的物质。
