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一种具有多个激发源的 5 公斤实时发光光度计。

A 5-kg time-resolved luminescence photometer with multiple excitation sources.

机构信息

U. S. Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center, 600 E. Mermaid Lane, Wyndmoor, Pennsylvania 19038, USA.

出版信息

Appl Spectrosc. 2012 Mar;66(3):341-6. doi: 10.1366/11-06483.

Abstract

A luminescence photometer was developed based on lanthanide-sensitized luminescence to detect environmental pollutants and residues in foods including, in particular, two classes of antibiotics: tetracyclines and fluoroquinolones. Multiple excitation sources, a xenon flashlamp and ultraviolet light-emitting diodes (UV LEDs), were used to their advantages. A photomultiplier tube module, gated to reject time-domain interferences, was used as a photodetector. Using danofloxacin as a model analyte, luminescence signal was linear in more than three decades (0.5-2000 ng/mL) with R(2) > 0.998 in each decade. This photometer achieved a limit of detection of 2.0 ng/mL with 1.3% average relative standard deviation. It is field deployable at 4.6 kg and 15 W power consumption.

摘要

研制了一种基于镧系元素敏化发光的荧光光度计,用于检测环境污染物和食品中的残留,包括两类抗生素:四环素类和氟喹诺酮类。利用氙闪光灯和紫外发光二极管(UV LED)作为多个激发源,各取其优势。采用光电倍增管模块作为光电探测器,门控以消除时域干扰。以丹诺沙星为模型分析物,在每十年内,发光信号在三个多数量级范围内(0.5-2000 ng/mL)呈线性,相关系数(R(2))均大于 0.998。该荧光光度计的检测限为 2.0 ng/mL,平均相对标准偏差为 1.3%。它在野外的重量为 4.6 千克,功耗为 15 瓦。

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