U. S. Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center, 600 E. Mermaid Lane, Wyndmoor, Pennsylvania 19038, USA.
Appl Spectrosc. 2012 Mar;66(3):341-6. doi: 10.1366/11-06483.
A luminescence photometer was developed based on lanthanide-sensitized luminescence to detect environmental pollutants and residues in foods including, in particular, two classes of antibiotics: tetracyclines and fluoroquinolones. Multiple excitation sources, a xenon flashlamp and ultraviolet light-emitting diodes (UV LEDs), were used to their advantages. A photomultiplier tube module, gated to reject time-domain interferences, was used as a photodetector. Using danofloxacin as a model analyte, luminescence signal was linear in more than three decades (0.5-2000 ng/mL) with R(2) > 0.998 in each decade. This photometer achieved a limit of detection of 2.0 ng/mL with 1.3% average relative standard deviation. It is field deployable at 4.6 kg and 15 W power consumption.
研制了一种基于镧系元素敏化发光的荧光光度计,用于检测环境污染物和食品中的残留,包括两类抗生素:四环素类和氟喹诺酮类。利用氙闪光灯和紫外发光二极管(UV LED)作为多个激发源,各取其优势。采用光电倍增管模块作为光电探测器,门控以消除时域干扰。以丹诺沙星为模型分析物,在每十年内,发光信号在三个多数量级范围内(0.5-2000 ng/mL)呈线性,相关系数(R(2))均大于 0.998。该荧光光度计的检测限为 2.0 ng/mL,平均相对标准偏差为 1.3%。它在野外的重量为 4.6 千克,功耗为 15 瓦。