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[细胞壁分级分离后含氮植物物质在鲁氏发酵罐中的降解动力学解释与预测]

[Explanation and prediction of the kinetics of degradation in a Rusitec fermentor of nitrogenous vegetable matter after cell wall fractionation].

作者信息

Blanchart G, Vignon B

机构信息

INRA, Laboratoire de la chaire de zootechnie de l'ENSAIA, Vandoeuvre-lès-Nancy, France.

出版信息

Reprod Nutr Dev. 1990;30(4):493-505.

PMID:2244963
Abstract

The relation between nitrogen solubility in Van Soest detergents and in vitro nitrogen degradation was studied on 11 substrates with degradation process calculations in digestive trials and an artificial semi-continuous flow (Rusitec) rumen. The kinetics of plant protein degradation in Rusitec, especially the maximum degradation values, were rather similar to those calculated from literature data obtained in situ. In the exponential model used to describe the protein degradation, the maximum disappearance (a + b) was negatively correlated with the percentage of acid detergent insoluble nitrogen in the Van Soest (ADIN/Total N). In the foods tested, the non degradable fraction corresponds to a quantity which is equivalent to approximately 76% of the ADIN fraction. The instantaneously degraded nitrogen (a) was rather well explained by the percentage of neutral detergent insoluble nitrogen in the Van Soest (NDIN/Total N). The rate of disappearance (c) of the slowly degraded fraction was inversely correlated with the proportion of ADF in the NDF, which demonstrates the latter's resistance to degradation. This type of fractionation could be used in predicting nitrogenous value of feed stuffs for ruminants.

摘要

在11种底物上研究了氮在范氏洗涤剂中的溶解度与体外氮降解之间的关系,并在消化试验和人工半连续流动(瘤胃模拟技术,Rusitec)瘤胃中进行了降解过程计算。瘤胃模拟技术中植物蛋白降解的动力学,尤其是最大降解值,与根据原位获得的文献数据计算得出的结果相当相似。在用于描述蛋白质降解的指数模型中,最大消失量(a + b)与范氏洗涤剂中酸性洗涤不溶性氮的百分比(ADIN/总氮)呈负相关。在所测试的食物中,不可降解部分相当于约76%的酸性洗涤不溶性氮部分的量。瞬时降解氮(a)可以很好地用范氏洗涤剂中中性洗涤不溶性氮的百分比(NDIN/总氮)来解释。缓慢降解部分的消失速率(c)与中性洗涤纤维(NDF)中酸性洗涤纤维(ADF)的比例呈负相关,这表明了后者对降解的抗性。这种分级方法可用于预测反刍动物饲料的含氮量。

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