Huntley J F, Mackellar A, Newlands G F, Irvine J, Miller H R
Moredun Research Institute, Edinburgh, United Kingdom.
APMIS. 1990 Oct;98(10):933-44. doi: 10.1111/j.1699-0463.1990.tb05018.x.
The distribution of the rat mast cell granule proteinases, rat mast cell proteinase I and II (RMCPI and II respectively) has been determined in rat tissues with the aid of highly sensitive and specific enzyme-linked immunosorbent assays (ELISA) and paired immunofluorescence. The major source of RMCPII is the gastrointestinal tract, although low concentrations were also detected in non-mucosal sites including thymus, mesenteric lymph nodes, liver, bone marrow, heart, kidney and spleen. Cellular localization by paired immunofluorescence showed that most cells contained either RMCPI or RMCPII, although a minor subpopulation in which individual cells contained both proteinases was also identified in a few tissues. RMCPII-containing cells predominated at mucosal surfaces but were also found in non-mucosal tissues. Individual cells expressing both RMCPI and II were present in lung, liver mesenteric lymph node and submucosa of stomach and were occasionally represented amongst serosal cells from the peritoneal cavity. Connective tissue mast cells of skin and tongue were identified as major sources of RMCPI, although this proteinase was widely distributed in all tissues examined. The present study demonstrates the heterogeneity of mast cell proteinase phenotypes in the rat and emphasises the difficulties in determining mast cell subtypes on tissue location alone.
借助高灵敏度和特异性的酶联免疫吸附测定（ELISA）及配对免疫荧光技术，已对大鼠组织中大鼠肥大细胞颗粒蛋白酶——大鼠肥大细胞蛋白酶I和II（分别为RMCPI和II）的分布情况进行了测定。RMCPII的主要来源是胃肠道，不过在包括胸腺、肠系膜淋巴结、肝脏、骨髓、心脏、肾脏和脾脏在内的非黏膜部位也检测到了低浓度的RMCPII。通过配对免疫荧光进行细胞定位显示，大多数细胞要么含有RMCPI，要么含有RMCPII，尽管在少数组织中也鉴定出了一小部分单个细胞同时含有这两种蛋白酶的亚群。含RMCPII的细胞在黏膜表面占主导地位，但在非黏膜组织中也有发现。同时表达RMCPI和II的单个细胞存在于肺、肝肠系膜淋巴结以及胃黏膜下层，偶尔也见于腹膜腔的浆膜细胞中。皮肤和舌的结缔组织肥大细胞被确定为RMCPI的主要来源，尽管这种蛋白酶在所有检测的组织中都广泛分布。本研究证明了大鼠肥大细胞蛋白酶表型的异质性，并强调了仅根据组织位置确定肥大细胞亚型的困难。
