Gibson S, Mackeller A, Newlands G F, Miller H R
Department of Medicine, Western General Hospital, Edinburgh, U.K.
Immunology. 1987 Dec;62(4):621-7.
The distribution of mast cell granule proteinases in the rat digestive system was determined immunohistochemically. The population of toluidine blue-staining mast cells was accounted for by cells containing either rat mast cell proteinase I (RMCPI) or rat mast cell proteinase II (RMCPII). Granules in greater than 90% of RMCPI-containing cells stained red after the Alcian blue/safranin sequence, whereas all RMCPII-containing mast cells stained blue. The red/RMCPI phenotype was typical of the connective tissue mast cells (CTMC) that populate the proximal, non-mucosal, regions of the digestive system, and was also abundant in the serosa and in rectal and gastric muscularis. The blue/RMCPII phenotype, absent from non-mucosal sites except for rare cells in intestinal submucosa and muscularis, predominated in all mucosal tissues and resembled mucosal mast cells (MMC). A third, minor population of cells containing RMCPI but staining blue in the Alcian blue/safranin sequence was detected in both non-mucosal and mucosal tissues. Blue/RMCPI mast cells were rare in the small intestine but more frequent in the mucosa of the stomach and large intestine and in the connective tissues. It is suggested that granule proteinase phenotyping may provide an alternative technique in the analysis of mast cell heterogeneity.
采用免疫组织化学方法测定大鼠消化系统中肥大细胞颗粒蛋白酶的分布。甲苯胺蓝染色的肥大细胞群体由含有大鼠肥大细胞蛋白酶I（RMCPI）或大鼠肥大细胞蛋白酶II（RMCPII）的细胞组成。在阿尔辛蓝/番红染色后，超过90%含RMCPI的细胞颗粒染成红色，而所有含RMCPII的肥大细胞染成蓝色。红色/RMCPI表型是结缔组织肥大细胞（CTMC）的典型特征，CTMC分布于消化系统近端的非黏膜区域，在浆膜、直肠和胃肌层中也很丰富。蓝色/RMCPII表型除在肠黏膜下层和肌层有罕见细胞外，在非黏膜部位不存在，在所有黏膜组织中占主导，类似于黏膜肥大细胞（MMC）。在非黏膜和黏膜组织中均检测到第三类少量细胞，它们含有RMCPI，但在阿尔辛蓝/番红染色序列中染成蓝色。蓝色/RMCPI肥大细胞在小肠中很少见，但在胃和大肠黏膜及结缔组织中更常见。提示颗粒蛋白酶表型分析可能为肥大细胞异质性分析提供一种替代技术。
