Division of Rheumatology, Department of Internal Medicine, University of Ulsan College of Medicine, Ulsan University Hospital, Ulsan 682-714, Korea.
Rheumatol Int. 2013 Feb;33(2):381-8. doi: 10.1007/s00296-012-2365-9. Epub 2012 Mar 27.
Accumulating evidence suggests that defects in the function of CD4(+)CD25(+) regulatory T cells (Tregs) are important in immune-mediated diseases such as rheumatoid arthritis. Here, we investigated the effects of various disease-modifying anti-rheumatic drugs (DMARDs) on Treg function. Tregs and CD4(+)CD25(-) effector T cells (Teffs) were isolated from peripheral blood mononuclear cells obtained from healthy adults. Isolated Tregs were cultured with the DMARDs methotrexate (MTX), sulfasalazine (SSZ), leflunomide (LEF), or infliximab (INF). We found that each DMARD had a different effect on Treg function. SSZ and LEF inhibited the anti-proliferative function of Tregs on cocultured Teffs and reduced Treg expression of Foxp3 mRNA, whereas MTX and INF did not.
越来越多的证据表明，CD4(+)CD25(+)调节性 T 细胞（Tregs）功能缺陷在类风湿关节炎等免疫介导的疾病中起重要作用。在这里，我们研究了各种疾病修饰抗风湿药物（DMARDs）对 Treg 功能的影响。从健康成年人外周血单核细胞中分离出 Tregs 和 CD4(+)CD25(-)效应 T 细胞（Teffs）。分离的 Tregs 与 DMARDs 甲氨蝶呤（MTX）、柳氮磺胺吡啶（SSZ）、来氟米特（LEF）或英夫利昔单抗（INF）一起培养。我们发现每种 DMARD 对 Treg 功能的影响不同。SSZ 和 LEF 抑制 Tregs 对共培养的 Teffs 的抗增殖作用，并降低 Treg Foxp3 mRNA 的表达，而 MTX 和 INF 则没有。
