BRD School of Biosciences, Sardar Patel University, Sardar Patel Maidan, Vadtal Road, Satellite Campus, Vallabh Vidyanagar, Gujarat, India.
Bioprocess Biosyst Eng. 2012 Oct;35(8):1343-53. doi: 10.1007/s00449-012-0723-3. Epub 2012 Mar 27.
Toxic waste generated by Jatropha seed cake after utilization of biodiesel on one hand has stimulated the need to develop new technologies to treat the waste and on the other, forced us to reevaluate the efficient utilization of its nutritive potential for production of various high-value compounds and its conversion to non-toxic forms which could be used as animal feed stock. In this study, Jatropha seed cake was used for production of cellulases by new isolate of Thermoascus aurantiacus under solid-state fermentation. The interaction of nitrogen source concentration, moisture ratio, initial pH of the medium and inoculum size was investigated and modelled using response surface methodology (RSM) using Box-Behnken Design (BBD). Under optimized conditions endo-β-1,4-glucanase, β-glucosidase and filter paper activities were found to be 124.44, 28.86, 4.87 U/g of substrate, respectively. Characterization of endo-β-1,4-glucanase, β-glucosidase was done after partial purification by ammonium sulfate fractionation followed by desalting. The endo-β-1,4-glucanase and β-glucosidase showed maximum activity at 70 °C and pH 4. Saccharification studies performed with different lignocellulosic substrates showed that sugar cane bagasse was most susceptible to enzymatic hydrolysis. The study suggests that Jatropha seed cake can be used as a viable nutrient source for cellulase production without any pretreatment under solid-state fermentation by T. aurantiacus.
利用生物柴油后，麻疯树种子饼产生的有毒废物一方面刺激了开发新技术来处理废物的需求，另一方面迫使我们重新评估其营养潜力的有效利用，以生产各种高价值化合物，并将其转化为无毒形式，可作为动物饲料。在这项研究中，利用新分离的橙色嗜热子囊菌在固态发酵下，由麻疯树种子饼生产纤维素酶。通过 Box-Behnken 设计（BBD）使用响应面法（RSM）研究并建模了氮源浓度、水分比、培养基初始 pH 值和接种量的相互作用。在优化条件下，内切-β-1,4-葡聚糖酶、β-葡萄糖苷酶和滤纸酶的活性分别为 124.44、28.86 和 4.87 U/g 底物。通过硫酸铵分级分离和脱盐对内切-β-1,4-葡聚糖酶和β-葡萄糖苷酶进行部分纯化后，对其进行了表征。内切-β-1,4-葡聚糖酶和β-葡萄糖苷酶在 70°C 和 pH4 时表现出最大活性。对不同木质纤维素底物进行糖化研究表明，甘蔗渣最易受到酶水解的影响。该研究表明，在固态发酵过程中，麻疯树种子饼无需任何预处理，就可以作为橙色嗜热子囊菌生产纤维素酶的可行营养源。
