Department of Clinical Immunology, Children's Hospital of Fudan University, Shanghai, China.
Acta Paediatr. 2012 Aug;101(8):e316-20. doi: 10.1111/j.1651-2227.2012.02679.x. Epub 2012 Apr 9.
The aim of the study was to evaluate the effects of hypothermia on neuron-specific enolase (NSE) and S-100 protein levels in cerebrospinal fluid (CSF) in neonates with hypoxic/ischaemic encephalopathy (HIE).
Fifty-one enrolled neonates with HIE were divided into two groups: hypothermia (n = 23) and control (n = 28). NSE and S-100 protein were measured with immunoradiometric assays. Amino acid neurotransmitters were also measured by reversed-phase high-performance liquid chromatography. Neurodevelopmental assessments were performed at 3 and 12 months of age.
Neuron-specific enolase and S-100 levels were lower, and neurodevelopment outcome was better in the hypothermia group compared with the control group. Among the infants who received hypothermia, CSF NSE and S-100 were significantly higher in those who developed severe neurological impairment (mental development index or physical development index <70). There were no significant differences between the two groups in amino acid neurotransmitters.
These results indicated that hypothermia was associated with decreased CSF NSE and S-100 level and correlated with neurodevelopmental outcome in infants with HIE.
本研究旨在评估低温对缺氧缺血性脑病（HIE）新生儿脑脊液（CSF）中神经元特异性烯醇化酶（NSE）和 S-100 蛋白水平的影响。
将 51 例 HIE 新生儿分为两组：低温组（n=23）和对照组（n=28）。采用免疫放射分析测定 NSE 和 S-100 蛋白水平。采用反相高效液相色谱法测定氨基酸神经递质。在 3 个月和 12 个月时进行神经发育评估。
与对照组相比，低温组 NSE 和 S-100 水平较低，神经发育结局较好。在接受低温治疗的婴儿中，CSF NSE 和 S-100 在发生严重神经功能障碍（精神发育指数或身体发育指数<70）的婴儿中明显升高。两组氨基酸神经递质无显著差异。
这些结果表明，低温与 HIE 新生儿 CSF 中 NSE 和 S-100 水平降低有关，并与神经发育结局相关。
