Instituto de Investigaciones Marinas, Consejo Superior de Investigaciones Científicas, 36208, Vigo, Spain.
Antonie Van Leeuwenhoek. 2012 Jun;102(1):187-91. doi: 10.1007/s10482-012-9726-y. Epub 2012 Mar 28.
A Gram-negative, aerobic, motile and slightly curved rod-shaped bacterium (BFLP-8(T)) was isolated from cutaneous mucus of wild long-snouted seahorses (Hippocampus guttulatus) captured in northwest Spain (Toralla, Galicia). Strain BFLP-8(T) grew at 10-35 °C and pH 5-9 (optimally at 25 °C and pH 7.0) and with 1-6 % (w/v) NaCl (optimally with 2 % NaCl). The predominant respiratory quinone (90 %) was ubiquinone with ten isoprene units (Q-10) and the major fatty acids identified were C(18:1) ω7c (54.8 % of the total), C(19:0) cyclo ω8c (11.6 %), C(16:0) (9.5 %), C(18:1) 2-OH (7.1 %) and C(16:1) ω11c (6.7 %). The G+C content of the DNA was 57.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain BFLP-8(T) formed a distinct clade within the family Sneathiellaceae but is not specifically associated with any species in the family. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain BFLP-8(T) represents a novel species within a new genus, for which the name Oceanibacterium hippocampi gen. nov., sp. nov. is proposed. The type strain is BFLP-8(T) (=CECT 7691(T) = DSM 23444(T)).
一株革兰氏阴性、需氧、运动且略微弯曲的杆状细菌(BFLP-8(T))从西班牙西北部捕获的野生长吻海马(Hippocampus guttulatus)的皮肤黏液中分离得到。BFLP-8(T) 菌株在 10-35°C 和 pH 5-9(最佳条件为 25°C 和 pH 7.0)以及 1-6%(w/v)NaCl(最佳条件为 2%NaCl)下生长。主要的呼吸醌(90%)是十异戊烯单元的泛醌(Q-10),鉴定出的主要脂肪酸是 C(18:1) ω7c(占总脂肪酸的 54.8%)、C(19:0) 环 ω8c(11.6%)、C(16:0)(9.5%)、C(18:1) 2-OH(7.1%)和 C(16:1) ω11c(6.7%)。DNA 的 G+C 含量为 57.8mol%。基于 16S rRNA 基因序列的系统发育分析表明,BFLP-8(T) 菌株在 Sneathiellaceae 科内形成一个独特的分支,但与该科内的任何物种都没有特定的关联。根据表型、化学分类和系统发育数据,BFLP-8(T) 菌株代表一个新属的新种,建议将其命名为 Oceanibacterium hippocampi 属,种。模式菌株为 BFLP-8(T)=CECT 7691(T)=DSM 23444(T)。