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海洋杆菌属(Oceanibacterium),新属,新种,从野生海马( Hippocampus guttulatus )的皮肤黏液中分离得到。

Oceanibacterium hippocampi gen. nov., sp. nov., isolated from cutaneous mucus of wild seahorses (Hippocampus guttulatus).

机构信息

Instituto de Investigaciones Marinas, Consejo Superior de Investigaciones Científicas, 36208, Vigo, Spain.

出版信息

Antonie Van Leeuwenhoek. 2012 Jun;102(1):187-91. doi: 10.1007/s10482-012-9726-y. Epub 2012 Mar 28.

Abstract

A Gram-negative, aerobic, motile and slightly curved rod-shaped bacterium (BFLP-8(T)) was isolated from cutaneous mucus of wild long-snouted seahorses (Hippocampus guttulatus) captured in northwest Spain (Toralla, Galicia). Strain BFLP-8(T) grew at 10-35 °C and pH 5-9 (optimally at 25 °C and pH 7.0) and with 1-6 % (w/v) NaCl (optimally with 2 % NaCl). The predominant respiratory quinone (90 %) was ubiquinone with ten isoprene units (Q-10) and the major fatty acids identified were C(18:1) ω7c (54.8 % of the total), C(19:0) cyclo ω8c (11.6 %), C(16:0) (9.5 %), C(18:1) 2-OH (7.1 %) and C(16:1) ω11c (6.7 %). The G+C content of the DNA was 57.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain BFLP-8(T) formed a distinct clade within the family Sneathiellaceae but is not specifically associated with any species in the family. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain BFLP-8(T) represents a novel species within a new genus, for which the name Oceanibacterium hippocampi gen. nov., sp. nov. is proposed. The type strain is BFLP-8(T) (=CECT 7691(T) = DSM 23444(T)).

摘要

一株革兰氏阴性、需氧、运动且略微弯曲的杆状细菌(BFLP-8(T))从西班牙西北部捕获的野生长吻海马(Hippocampus guttulatus)的皮肤黏液中分离得到。BFLP-8(T) 菌株在 10-35°C 和 pH 5-9(最佳条件为 25°C 和 pH 7.0)以及 1-6%(w/v)NaCl(最佳条件为 2%NaCl)下生长。主要的呼吸醌(90%)是十异戊烯单元的泛醌(Q-10),鉴定出的主要脂肪酸是 C(18:1) ω7c(占总脂肪酸的 54.8%)、C(19:0) 环 ω8c(11.6%)、C(16:0)(9.5%)、C(18:1) 2-OH(7.1%)和 C(16:1) ω11c(6.7%)。DNA 的 G+C 含量为 57.8mol%。基于 16S rRNA 基因序列的系统发育分析表明,BFLP-8(T) 菌株在 Sneathiellaceae 科内形成一个独特的分支,但与该科内的任何物种都没有特定的关联。根据表型、化学分类和系统发育数据,BFLP-8(T) 菌株代表一个新属的新种,建议将其命名为 Oceanibacterium hippocampi 属,种。模式菌株为 BFLP-8(T)=CECT 7691(T)=DSM 23444(T)。

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