反义介导的外显子跳跃以改变可变剪接来治疗癌症。
Antisense-mediated exon skipping to shift alternative splicing to treat cancer.
作者信息
Wan Jing
机构信息
University of North Carolina, Chapel Hill, NC, USA.
出版信息
Methods Mol Biol. 2012;867:201-8. doi: 10.1007/978-1-61779-767-5_13.
PMID:22454063
Abstract
Manipulation of alternative splicing is a unique way of regulating gene expression. Here, a detailed protocol is presented for modification of HER2 pre-mRNA alternative splicing. A 20-mer splice switching oligonucleotide (SSO) targeting exon 15 in HER2 pre-mRNA induced skipping of exon 15, resulted in significant downregulation of full-length HER2 mRNA and protein expression in HER2-overexpressing breast cancer cell line SK-BR-3.
摘要
调控可变剪接是调节基因表达的一种独特方式。在此，我们展示了一种用于修饰HER2前体mRNA可变剪接的详细方案。一种靶向HER2前体mRNA外显子15的20聚体剪接转换寡核苷酸（SSO）诱导外显子15跳跃，导致HER2过表达的乳腺癌细胞系SK-BR-3中全长HER2 mRNA和蛋白表达显著下调。
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