Bora Nagamani, Bawa Zharain, Bill Roslyn M, Wilks Martin D B
School of Life & Health Sciences and Aston Research Centre for Healthy Ageing, Aston University, Birmingham, UK.
Methods Mol Biol. 2012;866:115-27. doi: 10.1007/978-1-61779-770-5_11.
Biological processes are subject to the influence of numerous factors and their interactions, which may be non-linear in nature. In a recombinant protein production experiment, understanding the relative importance of these factors, and their influence on the yield and quality of the recombinant protein being produced, is an essential part of its optimisation. In many cases, implementing a design of experiments (DoE) approach has delivered this understanding. This chapter aims to provide the reader with useful pointers in applying a DoE strategy to improve the yields of recombinant yeast cultures.
生物过程受到众多因素及其相互作用的影响，这些因素本质上可能是非线性的。在重组蛋白生产实验中，了解这些因素的相对重要性及其对所生产重组蛋白产量和质量的影响，是优化实验的重要组成部分。在许多情况下，采用实验设计（DoE）方法可获得这种认识。本章旨在为读者提供一些有用的指导，介绍如何应用DoE策略提高重组酵母培养物的产量。
