家蝇蛹凝集素通过NF-κB/p65介导的半胱天冬酶途径诱导肝癌细胞HepG2凋亡。
Musca domestica pupae lectin induces apoptosis in HepG2 cells through a NF-κB/p65-mediated caspase pathway.
作者信息
Nie Jianzeng, Cao Xiaohong, Zhou Minghui, Zhang Xinyuan, Zhang Rongping, Niu Lili, Xia Yan, Hou Lihua, Wang Chunling
机构信息
Ministry of Education, Tianjin University of Science and Technology, College of Food Engineering and Biotechnology, Key Laboratory of Food Safety and Sanitation, Tianjin Economy Technological Development Area, China.
出版信息
Bull Cancer. 2012 Apr 1;99(4):E49-54. doi: 10.1684/bdc.2012.1557.
A new lectin (42 kDa) from Musca domestica pupae (MPL) has been known to inhibit proliferation in tumor cells. In this study, flow cytometry analysis showed that MPL induced HepG2 cells apoptosis significantly and the cells were arrested at S phase. MPL inhibited IκB-α degradation and NF-κB/p65 translocation from cytoplasm into nucleus. Simultaneously, the expressions of FLIP, which is a target gene of NF-κB/p65 were down-regulated and the caspase-8 and caspase-3 were then activated to induce apoptosis. Taken together, these results showed that MPL induced a caspase-dependent apoptosis via NF-κB/p65 pathway in HepG2 cells.
家蝇蛹中的一种新的凝集素（42 kDa）（MPL）已被证实可抑制肿瘤细胞增殖。在本研究中，流式细胞术分析表明，MPL可显著诱导HepG2细胞凋亡，且细胞停滞于S期。MPL抑制IκB-α降解以及NF-κB/p65从细胞质向细胞核的转位。同时，作为NF-κB/p65靶基因的FLIP表达下调，随后caspase-8和caspase-3被激活以诱导凋亡。综上所述，这些结果表明MPL通过NF-κB/p65途径在HepG2细胞中诱导了caspase依赖性凋亡。
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