家蝇蛹凝集素通过激活 NF-κB 抑制体外乙型肝炎病毒。
Inhibition on hepatitis B virus in vitro of lectin from Musca domestica pupa via the activation of NF-κB.
机构信息
Key Laboratory of Food Safety and Sanitation, Ministry of Education, College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, No. 29, 13th Avenue, Tianjin Economy Technological Development Area, Tianjin 300457, China.
出版信息
Virus Res. 2012 Dec;170(1-2):53-8. doi: 10.1016/j.virusres.2012.08.012. Epub 2012 Aug 23.
The present study reported that the secretions of HBsAg and HBeAg in HepG2.2.15 cells were significantly decreased under the treatment of lectin from Musca domestica pupa (MPL). Both the replication of hepatitis B virus (HBV) DNA and HBV cccDNA in cells, and the copies of extracellular HBV DNA were inhibited by MPL. The mRNA expressions of interleukin-2 (IL-2), gamma interferon (INF-γ) and MxA were up-regulated by MPL treatments, but down-regulated when nuclear factor-κB (NF-κB) signal pathway was blocked by pyrrolidine dithiocarbamate (PDTC). Subsequent investigation revealed that nuclear factor-κB inhibitory κB (IκB) in endochylema was inhibited and NF-κB was translocated into the nucleus. These findings indicate that MPL could inhibit HBV replication via the induction of the expression of IL-2, INF-γ and MxA through the activation of NF-κB.
本研究报道，槐果碱（MPL）处理可显著降低 HepG2.2.15 细胞中 HBsAg 和 HBeAg 的分泌。槐果碱可抑制乙型肝炎病毒（HBV）DNA 和 HBVcccDNA 在细胞中的复制，以及细胞外 HBV DNA 的拷贝数。槐果碱处理可上调白细胞介素-2（IL-2）、γ干扰素（INF-γ）和 MxA 的 mRNA 表达，但当核因子-κB（NF-κB）信号通路被吡咯烷二硫代氨基甲酸盐（PDTC）阻断时，其表达则下调。进一步研究表明，槐果碱可抑制细胞质中核因子-κB 抑制κB（IκB），并使 NF-κB 易位到细胞核。这些发现表明，槐果碱通过激活 NF-κB，诱导 IL-2、INF-γ 和 MxA 的表达，从而抑制 HBV 的复制。
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