Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok, Thailand.
Antonie Van Leeuwenhoek. 2012 Aug;102(2):221-9. doi: 10.1007/s10482-012-9728-9. Epub 2012 Mar 29.
Three strains (LM008(T), LM068 and LM078(T)), representing two novel yeast species were isolated from the phylloplane of three plant species by an enrichment technique. On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics, and the sequence analysis of the D1/D2 domain of the large subunit rRNA gene and the internal spacer region, the three strains were assigned as two novel Candida species. Strain LM008(T) was assigned to be Candida sirachaensis sp. nov. (type strain LM008(T) = BCC 47628(T) = NBRC 108605(T) CBS 12094(T)) in the Starmerella clade. Two strains (LM068 and LM078(T)) represent a single species in the Lodderomyces-Spathaspora clade for which the name Candida sakaeoensis sp. nov. is proposed with the type strain LM078(T) = BCC 47632(T) = NBRC 108895(T) = CBS 12318(T).
通过富集技术，从三种植物叶片上分离到三株（LM008(T)、LM068 和 LM078(T)）代表两个新型酵母物种的菌株。基于形态学、生物化学、生理学和化学分类学特征，以及大亚基 rRNA 基因的 D1/D2 结构域和内部间隔区的序列分析，这三株菌被归为两个新型的假丝酵母属物种。菌株 LM008(T)被分配为希拉辣味假丝酵母（Candida sirachaensis sp. nov.）（模式菌株 LM008(T)=BCC 47628(T)=NBRC 108605(T)=CBS 12094(T)），属于 Starmerella 分支。另外两株（LM068 和 LM078(T)）代表了 Lodderomyces-Spathaspora 分支中的一个单一物种，被命名为清酒假丝酵母（Candida sakaeoensis sp. nov.），模式菌株 LM078(T)=BCC 47632(T)=NBRC 108895(T)=CBS 12318(T)。
