Graduate School of Biomedical Engineering, University of New South Wales, Sydney, NSW 2052, Australia.
Biomed Mater. 2012 Apr;7(2):024106. doi: 10.1088/1748-6041/7/2/024106. Epub 2012 Mar 29.
The aim of this research was to understand the influence of functional group density on degradation and cell survival within injectable poly(vinyl alcohol) (PVA) hydrogels crosslinked through hydrazone bonds. For this purpose, PVA was modified with aldehyde and hydrazide functional groups. The click reaction between these two macromers, performed under physiologic conditions, led to hydrogel formation in less than 3 min. The influence of the crosslinking density on the gelation time, volumetric swelling ratio and mass loss of the hydrogels was investigated. These systems were slowly degradable as they maintained their gel-like state for more than 120 days. However, these networks also exhibited unusual degradation behaviour that could be the result of a breaking-forming bond phenomenon, attributable to the reversible nature of the hydrazone bond. This study also demonstrated that these networks maintained their mechanical strength while degrading, and cell encapsulation revealed the cytocompatibility of these systems.
本研究旨在探讨通过腙键交联的可注射聚乙烯醇（PVA）水凝胶中，官能团密度对降解和细胞存活的影响。为此，用醛基和酰肼基对 PVA 进行了修饰。在生理条件下，这两种大分子之间的点击反应在不到 3 分钟的时间内引发了水凝胶的形成。考察了交联密度对凝胶时间、体积溶胀比和水凝胶质量损失的影响。这些体系降解缓慢，因为它们在超过 120 天的时间内仍保持凝胶状态。然而，这些网络也表现出异常的降解行为，这可能是由于腙键的可逆性质导致的断键-形成键现象的结果。本研究还表明，这些网络在降解的同时保持了机械强度，细胞包封揭示了这些体系的细胞相容性。
