State Key Laboratory of Crop Stress Biology in Arid Areas, Key Laboratory of Crop Pest Integrated Pest Management on the Loess Plateau of Ministry of Agriculture, Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education, College of Plant Protection, Northwest A&F University, Yangling, 712100 Shaanxi, P. R. China.
Arch Virol. 2012 Jul;157(7):1261-7. doi: 10.1007/s00705-012-1294-y. Epub 2012 Mar 29.
Barley yellow dwarf viruses (BYDVs, mainly consisting of three strains, GAV, GPV and PAV, in China), barley stripe mosaic virus (BSMV), wheat yellow mosaic virus (WYMV), wheat dwarf virus (WDV) and wheat blue dwarf phytoplasma (WBD) constitute a group of major wheat pathogens that have caused huge yield losses but are scarcely distinguished by their phenotype alone. For the simultaneous detection and discrimination of these seven pathogens in wheat, a multiplex polymerase chain reaction (M-PCR) method was developed in this study through a series of parameter optimizations. Detection and sensitivity tests using samples collected from the field indicate that the M-PCR method can rapidly, simultaneously and relatively effectively detect BYDV-GAV, -GPV, -PAV, BSMV, WYMV, WDV and WBD.
大麦黄花叶病毒（BYDVs，在中国主要由 GAV、GPV 和 PAV 三种株系组成）、大麦条纹花叶病毒（BSMV）、小麦黄花叶病毒（WYMV）、小麦黄矮病毒（WDV）和小麦蓝矮植原体（WBD）是一组主要的小麦病原体，它们单独仅凭表型就已造成了巨大的产量损失，但却很难区分。为了同时检测和区分这七种病原体在小麦中的存在，本研究通过一系列参数优化，开发了一种多重聚合酶链反应（M-PCR）方法。使用从田间采集的样本进行的检测和灵敏度测试表明，M-PCR 方法可以快速、同时且相对有效地检测 BYDV-GAV、-GPV、-PAV、BSMV、WYMV、WDV 和 WBD。
