Department of Pharmaceutical Technology, Naresuan University, Phitsanulok, Thailand.
J Biomater Appl. 2013 Aug;28(2):175-86. doi: 10.1177/0885328212441847. Epub 2012 Mar 28.
In present study, the chitosan/starch/β-glycerol phosphate hydrogel was investigated as an effective carrier for chondrocytes and delivery of transforming growth factor-β1. In vitro study indicated that transforming growth factor-β1 was released sustainably for 14 days with its biological activity to stimulate chondrocyte functions, as indicated by the strong expression of type II collagen protein. Subcutaneous implantation to rats revealed the strong expressions of type II collagen and aggrecan messenger ribonucleic acids, and also type II collagen protein was observed in the hydrogel in combination with transforming growth factor-β1 within 2 weeks. Our collective results showed the potential of chitosan/starch/β-glycerol phosphate hydrogel for effective delivery of chondrocytes and transforming growth factor-β1, and preserve chondrocytes' phenotype and functions in vitro.
在本研究中，壳聚糖/淀粉/β-甘油磷酸水凝胶被用作软骨细胞的有效载体和转化生长因子-β1 的递送载体。体外研究表明，转化生长因子-β1 能够持续释放 14 天，其生物活性可刺激软骨细胞功能，如 II 型胶原蛋白的强烈表达所示。向大鼠皮下植入后，发现 II 型胶原和聚集蛋白信使核糖核酸的表达较强，并且在 2 周内还观察到水凝胶中与转化生长因子-β1 结合的 II 型胶原蛋白。我们的研究结果表明，壳聚糖/淀粉/β-甘油磷酸水凝胶具有有效递送软骨细胞和转化生长因子-β1 的潜力，并能在体外保持软骨细胞的表型和功能。
