Department of Molecular Biology and Microbiology, Tufts University, Boston, Massachusetts, USA.
J Virol. 2012 Jun;86(11):6341-4. doi: 10.1128/JVI.00083-12. Epub 2012 Mar 28.
We sought to determine the relationship between two recent additions to the murine leukemia virus (MLV) ecotropic subgroup: Mus cervicolor isolate M813 and Mus spicilegus endogenous retrovirus HEMV. Though divergent in sequence, the two viruses share an Env protein with similarly curtailed VRA and VRB regions, and infection by both is restricted to mouse cells. HEMV and M813 displayed reciprocal receptor interference, suggesting that they share a receptor. Expression of the M813 receptor murine sodium-dependent myo-inositol transporter 1 (mSMIT1) allowed previously nonpermissive cells to be infected by HEMV, indicating that mSMIT1 also serves as a receptor for HEMV. Our findings add HEMV as a second member to the MLV subgroup that uses mSMIT1 to gain entry into cells.
我们试图确定最近加入鼠白血病病毒（MLV）嗜性亚群的两种病毒之间的关系：M813 是来自沼泽麂的分离病毒，以及 HEMV 是来自帚尾豪猪的内源性逆转录病毒。尽管这两种病毒在序列上存在差异，但它们共享一个具有类似截短的 VRA 和 VRB 区域的 Env 蛋白，并且两者的感染都仅限于鼠细胞。HEMV 和 M813 显示出相互受体干扰，表明它们共享一个受体。表达 M813 受体鼠钠依赖性肌醇转运蛋白 1（mSMIT1）使以前非允许的细胞能够被 HEMV 感染，表明 mSMIT1 也作为 HEMV 的受体。我们的发现将 HEMV 添加为第二个使用 mSMIT1 进入细胞的 MLV 亚组成员。
