Department of Biological Chemistry, College of Bioscience and Biotechnology, Chubu University, 1200 Matsumoto-cho, Kasugai 487-8501, Japan.
J Agric Food Chem. 2012 May 2;60(17):4222-6. doi: 10.1021/jf2048542. Epub 2012 Apr 19.
Thermally assisted hydrolysis and methylation-gas chromatography (THM-GC) in the presence of trimethylsulfonium hydroxide, using a vertical microfurnace pyrolyzer, was validated for the compositional analysis of fatty acid components in microalgae. The chromatograms of a microalga, Pavlova lutheri , obtained under optimized THM conditions clearly showed a series of fatty acid methyl esters including thermally labile polyunsaturated fatty acid components. On the basis of these peak areas, their chemical compositions were rapidly determined without using any tedious sample pretreatment with a precision of <8% relative standard deviation. Moreover, the compositions thus obtained were in good agreement with those obtained by the conventional technique involving solvent extraction. Finally, the THM-GC technique was applied for the compositional analysis of fatty acid components in a newly found microalga, Coccomyxa gloeobotrydiformis . The obtained data showed a high abundance (24 mol %) of α-linolenic acid components, suggesting its potential usefulness as feed sources and/or functional foods.
在三甲基氢氧化硫存在下，采用垂直微炉热解器进行热辅助水解和甲基化-气相色谱（THM-GC），对微藻中脂肪酸成分的组成分析进行了验证。在优化的 THM 条件下获得的微藻 P. lutheri 的色谱图清楚地显示了一系列脂肪酸甲酯，包括热不稳定的多不饱和脂肪酸成分。基于这些峰面积，无需使用任何繁琐的样品预处理即可快速确定其化学成分，精密度小于 8%相对标准偏差。此外，由此获得的组成与通过涉及溶剂提取的常规技术获得的组成非常吻合。最后，THM-GC 技术应用于新发现的微藻 Coccomyxa gloeobotrydiformis 中脂肪酸成分的组成分析。获得的数据显示 α-亚麻酸成分含量高（24 mol%），表明其作为饲料来源和/或功能性食品具有潜在的用途。
