Faculty of Sciences, School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, Australia.
J Gerontol A Biol Sci Med Sci. 2012 Oct;67(10):1022-35. doi: 10.1093/gerona/glr263. Epub 2012 Mar 29.
There is accumulating evidence that mitochondrial respiratory malfunction is associated with aging-associated complex diseases. However, progress in our understanding of these diseases has been hampered by the sensitivity and throughput of systems employed to quantify dysfunction and inherent limitations of the biological systems studied. In this review, we describe and contrast two methodologies that have been developed for measuring mitochondrial function to address the need for improved sensitivity and increased throughput. We then consider the utility of each methodology in studying three biological systems: isolated mitochondria, cultured cells, and cell fibers and tissues. Finally, we discuss the application of each methodology in the study of mitochondrial dysfunction in Alzheimer's disease, type 2 diabetes mellitus, and aging-associated autophagy impairment and mitochondrial malfunction. We conclude that the methodologies are complementary, and researchers may need to examine multiple biological systems to unravel complex diseases of aging.
越来越多的证据表明，线粒体呼吸功能障碍与衰老相关的复杂疾病有关。然而，我们对这些疾病的理解进展受到所采用的定量功能障碍的系统的灵敏度和通量以及所研究的生物系统固有局限性的阻碍。在这篇综述中，我们描述并对比了两种已开发用于测量线粒体功能的方法，以解决提高灵敏度和增加通量的需求。然后，我们考虑了每种方法在研究三种生物系统中的效用：分离的线粒体、培养的细胞以及细胞纤维和组织。最后，我们讨论了每种方法在研究阿尔茨海默病、2 型糖尿病以及与衰老相关的自噬功能障碍和线粒体功能障碍中的线粒体功能障碍中的应用。我们的结论是，这些方法是互补的，研究人员可能需要检查多个生物系统来揭示衰老相关的复杂疾病。
