College of Animal Science and Veterinary Medicine, Jilin University, Changchun, China.
Theriogenology. 2012 Jul 1;78(1):39-48. doi: 10.1016/j.theriogenology.2012.01.016. Epub 2012 Mar 28.
The expression and localization of neurotrophin 4 (NT4) and its receptor, tyrosine kinase B (TRKB), in the bovine oviduct, and their interaction with gonadotrophins in bovine oviduct epithelial cells (BOECs), were examined. Transcripts for NT4 and TRKB were detected by reverse transcription polymerase chain reaction (RT-PCR) in bovine oviducts in the follicular and luteal phases, and their proteins were immunolocalized in BOECs. Based on real time PCR, NT4 mRNA did not differ significantly between the two phases of the cycle, although TRKB mRNA expression was higher (P < 0.05) in the luteal phase than that in follicular phase. The BOECs were treated with various concentrations of luteinizing hormone (LH) and follicle-stimulating hormone (FSH) in vitro; for NT4, mRNA and protein were higher (P < 0.05) than those in the control (based on real time PCR and enzyme-linked immunosorbent assay (ELISA) assays). The effects of NT4 and the TRKB inhibitor (K252a) on the expression of LH receptor (LHR) and FSH receptor (FSHR) in the oviduct epithelial cells were also studied using a monolayer culture model. Expression levels of LHR and FSHR mRNA in BOECs treated with various concentrations of NT4 were higher (P < 0.05) than those in the control. However, these expressions were blocked by treatment with K252α. We concluded that neurotrophin 4 may have a role in regulating the function of bovine oviducts by interacting with gonadotrophins.
神经生长因子 4（NT4）及其受体酪氨酸激酶 B（TRKB）在牛输卵管中的表达和定位，以及它们与牛输卵管上皮细胞（BOECs）中的促性腺激素的相互作用，进行了研究。通过反转录聚合酶链反应（RT-PCR）检测到牛输卵管在卵泡期和黄体期的 NT4 和 TRKB 转录本，并在 BOECs 中免疫定位其蛋白质。基于实时 PCR，NT4 mRNA 在两个周期阶段之间没有显著差异，尽管黄体期的 TRKB mRNA 表达（P <0.05）高于卵泡期。BOECs 用不同浓度的促黄体激素（LH）和促卵泡激素（FSH）体外处理；对于 NT4，mRNA 和蛋白质（基于实时 PCR 和酶联免疫吸附测定（ELISA）测定）高于对照（P <0.05）。还使用单层培养模型研究了 NT4 和 TRKB 抑制剂（K252a）对输卵管上皮细胞中 LH 受体（LHR）和 FSH 受体（FSHR）表达的影响。用不同浓度的 NT4 处理的 BOECs 中 LHR 和 FSHR mRNA 的表达水平（P <0.05）高于对照。然而，这些表达被 K252α 处理阻断。我们得出结论，神经生长因子 4 可能通过与促性腺激素相互作用在调节牛输卵管功能方面发挥作用。
