Li Wei, Sun Zhi-Qiang, Xie Ping, Dou Shuo-Xing, Wang Wei-Chi, Wang Peng-Ye
Key Laboratory of Soft Matter Physics, Beijing National Laboratory for Condensed Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing 100190, China.
Phys Rev E Stat Nonlin Soft Matter Phys. 2012 Feb;85(2 Pt 1):021918. doi: 10.1103/PhysRevE.85.021918. Epub 2012 Feb 22.
Magnetic tweezers were employed to investigate the interactions between DNA and cisplatin (transplatin). Cisplatin shortened DNA and reduced its persistence length more significantly than transplatin due to the formation of many more diadducts than those formed by transplatin. Interestingly, the presence of transplatin could enhance the ability of cisplatin in shortening DNA. An optimal concentration ratio of transplatin to cisplatin existed at which cisplatin showed the highest ability in shortening DNA. Moreover, abrupt length changes were also observed when DNA was treated with a mixture of cisplatin and transplatin at high concentrations. A model was proposed to qualitatively explain well these results.
采用磁镊研究DNA与顺铂（反铂）之间的相互作用。由于顺铂形成的二价加合物比反铂形成的多得多，所以顺铂比反铂更显著地缩短DNA并降低其持久长度。有趣的是，反铂的存在可以增强顺铂缩短DNA的能力。存在一个反铂与顺铂的最佳浓度比，在此浓度比下顺铂表现出最高的缩短DNA的能力。此外，当用高浓度的顺铂和反铂混合物处理DNA时，也观察到了长度的突然变化。提出了一个模型来定性地很好解释这些结果。
