Gallina M J, Kuchroo V K, Steele J K
Department of Immunology, Scripps Clinic & Research Institute, La Jolla, CA 92037.
J Immunol. 1990 Dec 1;145(11):3570-7.
A T cell hybridoma producing a T suppressor factor (TsF) with specificity for the hapten nitrophenyl was converted to long term growth in serum-free medium and its product tested by serology, bioactivity, and Western blot analysis. Results indicated that Ag-specific suppressive activity was present in serum-free medium and this TsF could exhibit the characteristics ascribed to it by various groups: it could bind nominal Ag with specificity, it was bound by anti-TsF mAb, and it could mediate Ag-specific suppression both in vivo and in vitro. Western blot and SDS-PAGE analysis of this purified TsF revealed a 43-kDa single chain protein.
一株产生对半抗原硝基苯基具有特异性的T抑制因子（TsF）的T细胞杂交瘤被转化为能在无血清培养基中长期生长，并通过血清学、生物活性和蛋白质免疫印迹分析对其产物进行检测。结果表明，无血清培养基中存在抗原特异性抑制活性，且这种TsF能够展现出不同研究团队所描述的特征：它能特异性结合名义抗原，可被抗TsF单克隆抗体结合，并且能在体内和体外介导抗原特异性抑制。对这种纯化的TsF进行蛋白质免疫印迹和SDS - 聚丙烯酰胺凝胶电泳分析，结果显示为一条43 kDa的单链蛋白。
