Steele J K, Kuchroo V K, Kawasaki H, Jayaraman S, Iwata M, Ishizaka K, Dorf M E
Department of Pathology, Harvard Medical School, Boston, MA 02115.
J Immunol. 1989 Apr 1;142(7):2213-20.
Five different Ag-binding suppressor factors from two types of hapten-specific Ts cell hybridomas (TsF1 inducer and TsF3 effector factors) were bound by an anti-lipomodulin mAb (141-B9), that crossreacts with rodent glycosylation inhibition factor (GIF). The Ag-specific suppressor activity in these hybridoma supernatants was bound by anti-lipomodulin columns and could be recovered by elution at acid pH. Additional evidence for the expression of lipomodulin/GIF activity on these TsF molecules was demonstrated by the ability of the eluted fractions to inhibit the glycosylation of IgE-binding peptides during their biosynthesis. The same biologic activity is associated with GIF and lipomodulin. The relationship between TsF and lipomodulin/GIF was confirmed in a serologic assay, which showed that TsF1 and TsF3 molecules, whether purified over Ag, anti-IJ or anti-TsF columns, are recognized by the mAb. 141-B9. The combined results indicate that Ag-binding Ts factors share a common antigenic determinant with phospholipase inhibitory proteins such as lipomodulin and GIF. In addition, the demonstration of glycosylation regulatory activity carried on these TsF molecules suggests a possible mode for their bioactivity.
来自两种半抗原特异性 Ts 细胞杂交瘤（TsF1 诱导因子和 TsF3 效应因子）的五种不同的抗原结合抑制因子被一种抗脂质调节蛋白单克隆抗体（141-B9）结合，该抗体与啮齿动物糖基化抑制因子（GIF）发生交叉反应。这些杂交瘤上清液中的抗原特异性抑制活性被抗脂质调节蛋白柱结合，并可通过在酸性 pH 下洗脱而回收。洗脱级分在 IgE 结合肽生物合成过程中抑制其糖基化的能力证明了这些 TsF 分子上存在脂质调节蛋白/GIF 活性的额外证据。相同的生物活性与 GIF 和脂质调节蛋白相关。在血清学试验中证实了 TsF 与脂质调节蛋白/GIF 之间的关系，该试验表明，无论在抗原柱、抗 IJ 柱或抗 TsF 柱上纯化，TsF1 和 TsF3 分子都能被单克隆抗体 141-B9 识别。综合结果表明，抗原结合 Ts 因子与磷脂酶抑制蛋白如脂质调节蛋白和 GIF 具有共同的抗原决定簇。此外，这些 TsF 分子上糖基化调节活性的证明提示了其生物活性的一种可能模式。
