Department of Cell Proliferation and Development, Centro de Investigaciones Biológicas (CSIC), Madrid, Spain.
Methods. 2012 Jun;57(2):170-8. doi: 10.1016/j.ymeth.2012.03.013. Epub 2012 Mar 24.
Two-dimensional (2D) agarose gel electrophoresis is one of the most powerful methods to analyze the mass and shape of replication intermediates. It is often use to map replication origins but it is also useful to characterize termination of replication, replication fork barriers and even replication fork reversal. Here, we present protocols, figures and movies with a thorough description of different modes of replication for linear DNA fragments and the corresponding patterns they generate in 2D gels.
二维（2D）琼脂糖凝胶电泳是分析复制中间体的质量和形状的最有力方法之一。它通常用于绘制复制起点，但也可用于表征复制终止、复制叉障碍，甚至复制叉反转。在这里，我们提供了带有详细描述的线性 DNA 片段不同复制模式的协议、图片和视频，以及它们在 2D 凝胶中产生的相应模式。
