Institute of Biochemistry and Molecular Biology, School of Life Sciences, Lanzhou University, 222 Tian Shui South Road, Lanzhou 730000, PR China.
Peptides. 2012 May;35(1):31-5. doi: 10.1016/j.peptides.2012.03.013. Epub 2012 Mar 23.
Neuronostatin is a 13-amino acid amidated peptide widely distributed in various organs including gastrointestinal tract. However, the effect of neuronostatin on gastrointestinal motility has not been well characterized. In the present work, effects of central administration of neuronostatin on gastric emptying and gastrointestinal transit were investigated. The results indicated that intracerebroventricular (i.c.v.) administration of neuronostatin (1, 5, 10 or 20nmol/mouse) delayed gastric emptying and gastrointestinal transit in a dose-related manner in mice. The effects were significantly reversed by melanocortin 3/4 receptor antagonist SHU9119 or classical opioid receptor antagonist naloxone, suggesting that the central melanocortin system and opioid system may be involved in the gastrointestinal effects elicited by i.c.v. administration of neuronostatin. In addition, we found that C-terminal amidation modification of neuronostatin is essential to exert its gastrointestinal effects. These results indicated that neuronostatin may play an important role in regulating gastrointestinal function.
神经元抑素是一种广泛分布于胃肠道等多种器官的 13 个氨基酸酰胺化肽。然而，神经元抑素对胃肠道动力的影响尚未得到很好的描述。在本工作中，研究了神经元抑素中枢给药对胃排空和胃肠转运的影响。结果表明，神经元抑素（1、5、10 或 20nmol/只）经脑室给药可剂量依赖性地延迟小鼠胃排空和胃肠转运。该作用可被黑素皮质素 3/4 受体拮抗剂 SHU9119 或经典阿片受体拮抗剂纳洛酮显著逆转，提示中枢黑素皮质素系统和阿片系统可能参与了神经元抑素脑室给药引起的胃肠道作用。此外，我们发现神经元抑素的 C 末端酰胺化修饰对于发挥其胃肠道作用是必需的。这些结果表明神经元抑素可能在调节胃肠道功能中发挥重要作用。
