Churchward Colin P, Hlúšek Miroslav, Nicholas Robin A J, Ayling Roger D, McAuliffe Laura
Mycoplasma Group, Department of Bacteriology, Animal Health and Veterinary Laboratories Agency, Surrey KT15 3NB, UK.
Vet Microbiol. 2012 Sep 14;159(1-2):257-9. doi: 10.1016/j.vetmic.2012.03.011. Epub 2012 Mar 13.
Mycoplasma mycoides subspecies mycoides small colony is the aetiological agent of contagious bovine pleuropneumonia, a cattle disease endemic to areas of sub-Saharan Africa. Twenty isolates from various geographical locations and the type strain were analysed by multi-locus sequence analysis (MLSA). The data generated was then used to develop three PCR primer sets to differentiate these isolates. The PCRs differentiated the isolates into four groups; the type strain (T); isolates of European origin (Eu); isolates from Tanzania (Af1) with a final group consisting of isolates from Namibia and Botswana (Af2). These PCRs offers a rapid and efficient post-identification typing method without the need to sequence and analyse multiple genes.
丝状支原体丝状亚种小菌落型是牛传染性胸膜肺炎的病原体，这种牛病在撒哈拉以南非洲地区流行。通过多位点序列分析（MLSA）对来自不同地理位置的20个分离株和模式菌株进行了分析。然后利用所产生的数据开发了三套PCR引物组，以区分这些分离株。PCR将这些分离株分为四组：模式菌株（T）；欧洲来源的分离株（Eu）；来自坦桑尼亚的分离株（Af1），最后一组由来自纳米比亚和博茨瓦纳的分离株组成（Af2）。这些PCR提供了一种快速有效的鉴定后分型方法，无需对多个基因进行测序和分析。
