Anantaworasakul P, Klayraung S, Okonogi S
Faculty of Pharmacy, Chiang Mai University, Chiang Mai, Thailand.
Drug Discov Ther. 2011 Feb;5(1):12-7. doi: 10.5582/ddt.v5.1.12.
In this study, Sesbania grandiflora, a plant in the Leguminosae family, was investigated for its antibacterial activities. The agar well diffusion assay as well as the agar and broth dilution assays were used for determination of antibacterial activities. The crude ethanolic extracts obtained from different parts of this plant exhibited different potent activities. The stem bark has the most potential to yield an extract with the highest antibacterial action. The fractionation of the stem bark with different solvents indicated that the fractionated extracts obtained from ethyl acetate or butanol possessed the most pronounced antibacterial activity. The kinetic study of bactericidal activities revealed that the butanol fractionated extract of the stem bark was effective against Gram negative bacteria. This study suggests that the stem bark of S. grandiflora contains promising antibacterial substances for clinical purposes.
在本研究中，对豆科植物大花田菁的抗菌活性进行了研究。采用琼脂孔扩散法以及琼脂和肉汤稀释法来测定抗菌活性。从该植物不同部位获得的粗乙醇提取物表现出不同的强效活性。茎皮最有可能产生具有最高抗菌作用的提取物。用不同溶剂对茎皮进行分级分离表明，从乙酸乙酯或丁醇中获得的分级提取物具有最显著的抗菌活性。杀菌活性的动力学研究表明，茎皮的丁醇分级提取物对革兰氏阴性菌有效。本研究表明，大花田菁的茎皮含有有前景的临床用抗菌物质。
