Mullins R, Wehry M, Hudgins R, Rink R
Department of Surgery, University of Louisville, Kentucky 40292.
J Surg Res. 1990 Nov;49(5):441-6. doi: 10.1016/0022-4804(90)90193-6.
Pyridoxalated polymerized hemoglobin (PPHG) has promise as a blood substitute for transfusing patients with hemorrhage. Exchange transfusion with PPHG depletes plasma proteins. The purpose of this study was to determine if, during the early repletion of intravascular proteins, albumin was transported from the interstitium of skin or skeletal muscle into the vascular compartment. PPHG was prepared from stroma-free human hemoglobin (100-120 mg/ml). The Hct of anesthetized rats dropped from 42 +/- 4% to 10 +/- 1% after exchange transfusion. Immediately postexchange plasma albumin declined from 24 +/- 2 to 6 +/- 3 mg/ml. Five hours postexchange transfusion plasma albumin had doubled and the skin and skeletal muscle albumin content was 80% of control. These data indicate that a shift of interstitial albumin from skin and skeletal muscle can rapidly replace plasma protein deficits after massive transfusion with PPHG.
吡哆醛聚合血红蛋白（PPHG）有望作为一种血液替代品用于给出血患者输血。用PPHG进行换血会消耗血浆蛋白。本研究的目的是确定在血管内蛋白质早期补充过程中，白蛋白是否从皮肤或骨骼肌间质转运到血管腔。PPHG由无基质人血红蛋白（100 - 120 mg/ml）制备。换血后麻醉大鼠的血细胞比容从42±4%降至10±1%。换血后即刻血浆白蛋白从24±2降至6±3 mg/ml。换血后5小时血浆白蛋白翻倍，皮肤和骨骼肌白蛋白含量为对照的80%。这些数据表明，在大量输注PPHG后，皮肤和骨骼肌间质白蛋白的转移可迅速弥补血浆蛋白不足。
