电针“丰隆”（ST 40）对高脂血症大鼠的影响及其机制

[Effect of electroacupuncture at "Fenglong" (ST 40) on rats with hyperlipidemia and its mechanism].

作者信息

Zhang Hong-Xing, Wang Qiong, Huang Hao, Yue Wei, Qin Peng-Fei

机构信息

Wuhan Hospital of Integrated Chinese and Western Medicine, The Affiliated Hospital of Hubei University of CM, Wuhan 430022, China.

出版信息

Zhongguo Zhen Jiu. 2012 Mar;32(3):241-5.

PMID:22471141

Abstract

OBJECTIVE

To explore the mechanism of electroacupuncture(EA) at "Fenglong" (ST 40) in hyperlipidemia (HLP) rats.

METHODS

Forty health SD rats were randomly divided into a normal control group (group A), a high fat forage feed group (group B) and a high fat forage feed treatment group (group C), a high fat forage + normal forage feed group (group D) and a high fat forage + normal forage feed treatment group (group E), eight rats in each group. EA was applied at "Fenglong" (ST 40) for the rats in group C and group E, once daily. After treatment of 30 days, blood lipid levels of rats, including total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C) in plasma were tested. Real time polymerase chain reaction (RT-PCR) and Western Blotting were applied to detect the gene expression changes of the contents of ATP-binding cassette transporter A1 (ABCA1), peroxisome proliferator-activated receptor alpha (PPARalpha), liver X receptor alpha (LXR-alpha) and retinoid X receptor alpha (RXR-alpha) in liver tissue of rats.

RESULTS

Compared with group A, the contents of TC, LDL-C were significantly elevated in group B and group D (all P < 0.01); compared with group B, above indices were significantly decreased in group D (all P < 0.01). After the treatment of EA at "Fenglong"(ST 40), the contents of TC, LDL-C were significantly decreased (all P < 0.01), and the contents of TG, HDL-C did not change obviously (all P > 0.05). Compared with group A, the mRNA and protein contents of ABCA1, PPARalpha, LXR-alpha and RXR-alpha were decreased obviously in group B and group D (all P < 0.01). But compared with group B, the above indices were decreased in the group D. There were signficantly increasing in the protein content of ABCA1, PPARalpha, RXR-alpha and LXR-alpha mRNA after the treatment of EA (all P < 0.05).

CONCLUSION

EA at "Fenglong" (ST 40) has some therapeutic effect on decrease the content of TC, LDL-C in rats of hyperlipemia and improve the gene expression of ABCA1, PPARalpha, LXR-alpha and RXR-alpha mRNA so as to promote reverse cholesterol transport.

摘要

目的

探讨电针“丰隆”（ST40）对高脂血症（HLP）大鼠的作用机制。

方法

将40只健康SD大鼠随机分为正常对照组（A组）、高脂饲料组（B组）、高脂饲料治疗组（C组）、高脂饲料+正常饲料组（D组）和高脂饲料+正常饲料治疗组（E组），每组8只。对C组和E组大鼠针刺“丰隆”（ST40），每日1次。治疗30天后，检测大鼠血浆总胆固醇（TC）、甘油三酯（TG）、低密度脂蛋白胆固醇（LDL-C）、高密度脂蛋白胆固醇（HDL-C）水平。采用实时荧光定量聚合酶链反应（RT-PCR）和蛋白质免疫印迹法（Western Blotting）检测大鼠肝组织中ATP结合盒转运体A1（ABCA1）、过氧化物酶体增殖物激活受体α（PPARα）、肝X受体α（LXR-α）和视黄醇X受体α（RXR-α）含量的基因表达变化。

结果

与A组比较，B组和D组大鼠TC、LDL-C含量显著升高（均P<0.01）；与B组比较，D组上述指标显著降低（均P<0.01）。电针“丰隆”（ST40）治疗后，大鼠TC、LDL-C含量显著降低（均P<0.01），TG、HDL-C含量无明显变化（均P>0.05）。与A组比较，B组和D组大鼠ABCA1、PPARα、LXR-α和RXR-α的mRNA和蛋白含量明显降低（均P<0.01）。但与B组比较，D组上述指标降低。电针治疗后ABCA1、PPARα、RXR-α蛋白含量及LXR-α mRNA显著升高（均P<0.05）。