电针刺激“丰隆”（ST 40）对高脂血症大鼠肝脏ATP结合盒转运蛋白A1 mRNA和蛋白表达的影响

[Effects of electroacupuncture stimulation of "Fenglong" (ST 40) on expression of liver ATP-binding cassette transporter A 1 mRNA and protein in rats with hyperlipidemia].

作者信息

Zhang Hong-Xing, Wu Huan, Huang Hao, Xiao Ying, Wang Dong

机构信息

Wuhan Integrated Chinese and Western Medicine Hospital, Wuhan, China.

出版信息

Zhen Ci Yan Jiu. 2013 Apr;38(2):100-5.

PMID:23819210

Abstract

OBJECTIVE

To observe the effect of electroacupuncture (EA) at "Fenglong" (ST 40) on blood lipid levels and hepatic ATP binding cassette transporter A 1(ABCA 1) mRNA and protein expression in hyperlipidemia rats, so as to study its mechanism underlying improvement of HLP.

METHODS

Forty SD rats were randomized into normal control, model,diet control, EA, and EA+ diet control groups, with 8 rats in each group. Hyperlipidemia model was established by feeding the animals with high fat forage for 28 days. After modeling, rats of the two diet control groups were fed with basal forage. EA (2 mA, 2 Hz/ 100 Hz) was applied to bilateral ST 40 for 30 min, once daily for 28 days. Plasma total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), and high-density lipoprotein cholesterol (HDL-C) contents were detected by automatic biochemistry analyzer, and the expression levels of ABCA 1 mRNA and protein in the liver tissue were assayed by in situ hybridization(lSH), RT-PCR and Western blot, respectively.

RESULTS

Compared with the normal control group, the contents of plasma TC and LDL-C were apparently increased (P<0.01) and the expression levels of hepatic ABCA 1 mRNA (detected by both ISH and RT-PCR) and ABCA 1 protein were significantly decreased in the model group (P<0.01). In comparison with the model group, plasma TC and LDL-C contents were significantly decreased and the expression levels of hepatic ABCA 1 mRNA and protein were significantly up-regulated in the EA group and EA+diet control group( P<0. 01). The effects of EA+ diet control were significantly superior to those of diet control in down-regulating plasma TC and LDL-C levels and up-regulating hepatic ABCA 1 mRNA and protein expression levels(P<0. 01).

CONCLUSION

EA stimulation of "Fenglong"(ST 40) can decrease the load of blood cholesterol and suppress the down-regulation of hepatic ABCA 1 mRNA and protein expression in hyperlipidemia rats, which may contribute to its effect in improving hyperlipidemia.

摘要

目的

观察电针“丰隆”（ST40）对高脂血症大鼠血脂水平及肝脏三磷酸腺苷结合盒转运体A1（ABCA1）mRNA和蛋白表达的影响，以探讨其改善高脂血症的作用机制。

方法

将40只SD大鼠随机分为正常对照组、模型组、饮食控制组、电针组和电针+饮食控制组，每组8只。采用高脂饲料喂养动物28天建立高脂血症模型。造模后，两个饮食控制组的大鼠喂以基础饲料。电针（2 mA，2 Hz/100 Hz）双侧ST40 30分钟，每日1次，共28天。用自动生化分析仪检测血浆总胆固醇（TC）、甘油三酯（TG）、低密度脂蛋白胆固醇（LDL-C）和高密度脂蛋白胆固醇（HDL-C）含量，分别采用原位杂交（ISH）、逆转录-聚合酶链反应（RT-PCR）和蛋白质免疫印迹法检测肝组织中ABCA1 mRNA和蛋白的表达水平。

结果

与正常对照组比较，模型组大鼠血浆TC和LDL-C含量明显升高（P<0.01），肝脏ABCA1 mRNA（ISH和RT-PCR检测）和ABCA1蛋白表达水平明显降低（P<0.01）。与模型组比较，电针组和电针+饮食控制组大鼠血浆TC和LDL-C含量明显降低，肝脏ABCA1 mRNA和蛋白表达水平明显上调（P<0.01）。电针+饮食控制组在下调血浆TC和LDL-C水平及上调肝脏ABCA1 mRNA和蛋白表达水平方面的作用明显优于饮食控制组（P<0.01）。