Arya Shashi K, Roy B K
Centre of Advanced Study in Botany, Banaras Hindu University, Varanasi, 221 005, India
J Environ Biol. 2011 Nov;32(6):707-11.
The effect of manganese (Mn) on broad bean (Vicia faba L.) was studied with regard to growth, Mn accumulation in root and shoot, chlorophyll, proline content and peroxidase activity. Seeds were treated with Mn (10, 20, 40, 80,120,160 microM) and grown hydroponically up to 15 days. Manganese level in both root and shoot increased progressively in response to increasing concentration and it was high in roots (13 fold) overthe shoots (8 fold). The reductions in root (52%) and shoot (62.92%) development were evident for the maximum Mn concentration (160 microM). The chlorophyll amount gradually declined with increasing Mn concentrations and attained its maximum (42%) at 160 microM. By contrast, the guaiacol peroxidase activity was high (71%) along with the accompanying rise in proline content (75%) in shoots of the highest Mn concentration (160 microM). However, there was about 2 fold increase in total glutathione content at 40 microM than the basal level and further declined to 21.65 microg g(-1) fresh wt. at 160 microM Mn. The alterations in overall reflected Mn concentration-dependent changes in the parameters studied. The results suggest thatthe plant Vicia faba L. copes with Mn exposure through enhanced production of antioxidants.
研究了锰（Mn）对蚕豆（Vicia faba L.）生长、根和地上部锰积累、叶绿素、脯氨酸含量及过氧化物酶活性的影响。种子用锰（10、20、40、80、120、160微摩尔）处理，水培生长15天。随着浓度增加，根和地上部的锰含量均逐渐增加，且根中的锰含量（13倍）高于地上部（8倍）。在最高锰浓度（160微摩尔）下，根（52%）和地上部（62.92%）的生长明显受到抑制。叶绿素含量随着锰浓度的增加而逐渐下降，在160微摩尔时达到最大值（42%）。相比之下，在最高锰浓度（160微摩尔）的地上部，愈创木酚过氧化物酶活性较高（71%），同时脯氨酸含量也随之增加（75%）。然而在40微摩尔时，总谷胱甘肽含量比基础水平增加了约2倍，在160微摩尔锰时进一步降至21.65微克/克鲜重。总体变化反映了所研究参数随锰浓度的变化。结果表明，蚕豆通过增强抗氧化剂的产生来应对锰暴露。
