Pandey Sanjeev, Saha Pradipta, Biswas Subinoy, Maiti Tushar K
Banwarilal Bhalotia College, Asansol, West Bengal, 713 303, India.
J Environ Biol. 2011 Nov;32(6):773-9.
Two strains of Bacillus sp. resistant to arsenate and lead designated as AsSP9 and PbSP6, respectively were isolated from the slag disposal site. They were identified to be related to Bacillus cereus cluster on the basis of 16S rDNA based sequence analysis and phenotypic characteristics. Both were rod-shaped (AsSP9, 2-5 microm and PbSP6, 2-4 microm), aerobic, salt tolerant (2-8% NaCI), endospore forming bacteria with minor differences like the AsSP9 showed sporangial bulging and PbSP6 had positive lipase activity. The temperature range for their growth was 20-40 degrees C and pH range 6.0-9.0 with an optimum temperature of 37 degrees C and pH of 7 for both strains. The principal nitrogen sources forAsSP9 and PbSP6 were DL-Tryptophan and L-Phenylalanine, respectively. The suitable carbon source forAsSP9 was lactose and for PbSP6 sucrose. The heavy metal accumulation efficiency was found to be 0.0047 mg g(-1) of dry mass forAsSP9 and 0.686 mg g(-1) of dry mass for PbSP6.
从矿渣处理场分别分离出两株对砷酸盐和铅具有抗性的芽孢杆菌菌株，分别命名为AsSP9和PbSP6。基于16S rDNA序列分析和表型特征，它们被鉴定与蜡样芽孢杆菌群相关。二者均为杆状（AsSP9，2 - 5微米；PbSP6，2 - 4微米），需氧，耐盐（2 - 8%氯化钠），可形成芽孢的细菌，存在一些细微差异，如AsSP9显示出芽孢囊膨胀，PbSP6具有阳性脂肪酶活性。它们的生长温度范围为20 - 40℃，pH范围为6.0 - 9.0，两株菌株的最适温度均为37℃，最适pH均为7。AsSP9和PbSP6的主要氮源分别是DL - 色氨酸和L - 苯丙氨酸。AsSP9的合适碳源是乳糖，PbSP6的合适碳源是蔗糖。发现AsSP9的重金属积累效率为0.0047毫克/克干重，PbSP6为0.686毫克/克干重。
