Dou Guiming, Liu Hongcan, He Wei, Ma Yuchao
College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, 100083, People's Republic of China.
College of Forestry, Beijing Forestry University, Beijing, 100083, People's Republic of China.
Antonie Van Leeuwenhoek. 2016 Jan;109(1):149-58. doi: 10.1007/s10482-015-0616-y. Epub 2015 Nov 24.
Two alkaliphilic and halotolerant Gram-stain positive, rod-shaped and endospore-forming bacteria, designated strains 12-3(T) and 12-4, were isolated from saline and alkaline soils collected in Lindian county, Heilongjiang province, China. Both strains were observed to grow well at a wide range of temperature and pH values, 10-45 °C and pH 8-12, with optimal growth at 37 °C and pH 9.0, respectively. Growth of the two strains was found to occur at total salt concentrations of 0-12 % (w/v), with an optimum at 4 % (w/v). The G+C contents of the genomic DNA of strains 12-3(T) and 12-4 were determined to be 42.7 and 42.4 mol%, respectively, and the major cellular fatty acids were identified as anteiso-C15:0 and anteiso-C17:0. In isolate 12-3(T), meso-diaminopimelic acid was found to be the diagnostic diamino acid of the cell wall peptidoglycan; diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol were identified as the major cellular polar lipids; and menaquinone-7 was identified as the predominant isoprenoid quinone. Strains 12-3(T) and 12-4 share very close 16S rRNA gene sequence similarity (99.74 %) and their DNA-DNA relatedness was 95.3 ± 0.63 %, meaning that the two strains can be considered to belong to the same species. 16S rRNA gene sequence-based phylogenetic analysis revealed strains 12-3(T) and 12-4 exhibit high similarities to Bacillus pseudofirmus DSM 8715(T) (98.7 %), Bacillus marmarensis DSM 21297(T) (97.2 %) and Bacillus nanhaiisediminis CGMCC 1.10116(T) (97.1 and 97.0 %, respectively). DNA-DNA hybridization values between isolate 12-3(T) and the type strains of closely related Bacillus species were below 30 %. On the basis of the polyphasic evidence presented, strains 12-3(T) and 12-4 are considered to represent a novel species of the genus Bacillus, for which the name Bacillus lindianensis sp. nov. is proposed. The type strain is 12-3(T) (DSM 26864(T) = CGMCC 1.12717(T)).
从中国黑龙江省林甸县采集的盐碱土中分离出两株嗜碱耐盐的革兰氏染色阳性、杆状且形成芽孢的细菌，分别命名为菌株12 - 3(T)和12 - 4。观察发现，两株菌在较宽的温度和pH值范围内生长良好，温度为10 - 45℃，pH值为8 - 12，最适生长温度分别为37℃，最适pH值为9.0。两株菌在总盐浓度为0 - 12%（w/v）时均可生长，最适盐浓度为4%（w/v）。菌株12 - 3(T)和12 - 4基因组DNA的G + C含量分别测定为42.7和42.4 mol%，主要细胞脂肪酸鉴定为anteiso - C15:0和anteiso - C17:0。在分离株12 - 3(T)中，发现内消旋二氨基庚二酸是细胞壁肽聚糖的诊断性二氨基酸；双磷脂酰甘油、磷脂酰乙醇胺和磷脂酰甘油被鉴定为主要细胞极性脂质；甲基萘醌 - 7被鉴定为主要类异戊二烯醌。菌株12 - 3(T)和12 - 4的16S rRNA基因序列相似度非常高（99.74%），它们的DNA - DNA相关性为95.3±0.63%，这意味着这两株菌可被认为属于同一物种。基于16S rRNA基因序列的系统发育分析表明，菌株12 - 3(T)和12 - 4与类芽孢杆菌DSM 8715(T)（98.
