Khoja Kholoud K, Shaf Gowhar, Hasan Tarique N, Syed Naveed Ahmed, Al-Khalifa Abdrohman S, Al-Assaf Abdullah H, Alshatwi Ali A
Dept. of Food Sciences and Nutrition, King Saud University, Riyadh, Saudi Arabia.
Asian Pac J Cancer Prev. 2011;12(12):3299-304.
There is growing use of anticancer complementary and alternative medicines worldwide. Trigonella foenum graecum (Fenugreek) is traditionally applied to treat disorders such as diabetes, high cholesterol, wounds, inflammation, and gastrointestinal ailments. Fenugreek is also reported to have anticancer properties due to its active beneficial chemical constituents. The mechanism of action of several anticancer drugs is based on their ability to induce apoptosis. The objective of the study was to characterize the downstream apoptotic genes targeted by FCE in MCF-7 human immortalized breast cells. FCE effectively killed MCF-7 cells through induction of apoptosis,confirmed by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) and RT-PCR assays. When cells were exposed to 50 μg/mL FCE for 24 hours, 23.2% apoptotic cells resulted, while a 48-hour exposure to 50 μg/mL caused 73.8% apoptosis. This was associated with increased expression of Caspase 3, 8, 9, p53, Fas, FADD, Bax and Bak in a time-and dose-dependent manner, as determined by real- time quantitative PCR. In summary, the induction of apoptosis by FCE is effected by its ability to increase the expression of pro-apoptotic genes and the spice holds promise for consideration in complementary therapy for breast cancer patients.
全球范围内，抗癌补充和替代药物的使用越来越多。胡芦巴传统上用于治疗糖尿病、高胆固醇、伤口、炎症和胃肠道疾病等病症。据报道，胡芦巴因其活性有益化学成分也具有抗癌特性。几种抗癌药物的作用机制基于它们诱导细胞凋亡的能力。本研究的目的是鉴定胡芦巴提取物（FCE）在MCF-7人永生化乳腺细胞中靶向的下游凋亡基因。FCE通过诱导细胞凋亡有效杀死MCF-7细胞，这通过末端脱氧核苷酸转移酶介导的dUTP缺口末端标记（TUNEL）和RT-PCR分析得到证实。当细胞暴露于50μg/mL FCE 24小时时，产生了23.2%的凋亡细胞，而暴露于50μg/mL 48小时导致73.8%的细胞凋亡。通过实时定量PCR测定，这与半胱天冬酶3、8、9、p53、Fas、FADD、Bax和Bak的表达以时间和剂量依赖性方式增加有关。总之，FCE诱导细胞凋亡是通过其增加促凋亡基因表达的能力实现的，这种香料有望用于乳腺癌患者的辅助治疗。
