Peng Chuanliang, Ren Peng, Zhou Qingling, Sun Qifeng, Zhao Xiaogang, Cong Bo
Department of the Second Hospital of Shandong University, Jinan, China.
Asian Pac J Cancer Prev. 2011;12(12):3371-4.
Heparanase (HPSE), as the only enzyme which can degrade the extracellular matrix and heparin sulfate in basement membrane, plays an important role in invasion and metastasis of tumor cells. In this study, we evaluated the inhibitory effect of HPSE antisense oligoxydeonucleotide (ASODN) on lung carcinoma cell line A-549 invasion.
Liposome-mediated ASODN was transfected into A-549 cells and expression of HPSE protein and mRNA were detected by flow cytometry and RT-PCR techniques. Matrigel invasion assays were employed to assess effects on invasiveness.
Lower expression of HPSE protein and mRNA and lower invasive ability to recombinate basal membrane were apparent after ASOND treatment (P<0.01). The inhibition rates of cell invasiveness were 55.6%, 82.3% and 91.2% treated by ASODN at final concentrations of 100, 200 and 400nmol/L, respectively.
HPSE ASODN can downregulate the expression of HPSE protein and mRNA in the A-549 cell line and can obviously inhibit its invasive ability in a dose-dependent manner in vitro.
乙酰肝素酶（HPSE）作为唯一能够降解细胞外基质和基底膜中硫酸乙酰肝素的酶，在肿瘤细胞的侵袭和转移中发挥重要作用。在本研究中，我们评估了HPSE反义寡脱氧核苷酸（ASODN）对肺癌细胞系A-549侵袭的抑制作用。
采用脂质体介导将ASODN转染至A-549细胞，通过流式细胞术和RT-PCR技术检测HPSE蛋白和mRNA的表达。采用基质胶侵袭实验评估对侵袭能力的影响。
ASOND处理后，HPSE蛋白和mRNA表达降低，对重组基底膜的侵袭能力降低（P<0.01）。终浓度为100、200和400nmol/L的ASODN处理后，细胞侵袭抑制率分别为55.6%、82.3%和91.2%。
HPSE ASODN可下调A-549细胞系中HPSE蛋白和mRNA的表达，并在体外以剂量依赖方式明显抑制其侵袭能力。
