剪接体的结构。

Architecture of the spliceosome.

机构信息

Department of Biochemistry, Brandeis University, 415 South Street, Waltham, MA 02454, USA.

出版信息

Biochemistry. 2012 Apr 24;51(16):3321-33. doi: 10.1021/bi201215r. Epub 2012 Apr 10.

DOI:10.1021/bi201215r

Abstract

Precursor-mRNA splicing is catalyzed by an extraordinarily large and highly dynamic macromolecular assemblage termed the spliceosome. Detailed biochemical and structural study of the spliceosome presents a formidable challenge, but there has recently been significant progress made on this front highlighted by the crystal structure of a 10-subunit human U1 snRNP. This review provides an overview of our current understanding of the architecture of the spliceosome and the RNA-protein complexes integral to its function, the U snRNPs.

摘要

前体 mRNA 的剪接是由一个称为剪接体的非常大且高度动态的大分子组装体催化的。对剪接体的详细生化和结构研究提出了一个艰巨的挑战，但最近在这方面取得了重大进展，突出表现在人 U1 snRNP 的 10 个亚基晶体结构的解析。本文综述了我们目前对剪接体的结构以及与其功能相关的 RNA-蛋白质复合物（U 小核核糖核蛋白）的认识。