Alam Iftikhar, Goldeck David, Larbi Anis, Pawelec Graham
Tübingen Aging and Tumour Immunology Group, Sektion für Transplantationsimmunologie und Immunohämatologie, University of Tübingen, Zentrum für MedizinischeForschung, Tübingen, Germany.
J Immunoassay Immunochem. 2012;33(2):128-39. doi: 10.1080/15321819.2011.604370.
Multicenter immune monitoring programs commonly rely on storing and shipping cryopreserved peripheral blood mononuclear cells (PBMC), isolated from whole blood before freezing. However, under many conditions in the field, facilities to separate PBMC are absent. Here, we investigate the feasibility of using whole blood (WB) frozen at -80°C as a source of viable lymphocytes for use in immunological studies. We compare the percentage of CD4 and CD8 T lymphocytes and their subsets from frozen WB with results from cryopreserved PBMC in five random healthy blood donors (three female, two male). We report that CD4 and CD8 values in lymphocytes from WB frozen up to 120 days were very similar to those of PBMC frozen up to 10 days. These data suggest that within the limits of parameters investigated in this study, contrary to our original assumptions, whole blood frozen at -80°C may in fact be an appropriate source of viable lymphocytes for T cell enumeration assays in immunological and epidemiological studies.
多中心免疫监测项目通常依赖于储存和运输冷冻保存的外周血单个核细胞（PBMC），这些细胞是在冷冻前从全血中分离出来的。然而，在许多实地条件下，缺乏分离PBMC的设施。在此，我们研究了将保存在-80°C的全血（WB）作为用于免疫学研究的活淋巴细胞来源的可行性。我们比较了五名随机选取的健康献血者（三名女性，两名男性）冷冻WB中CD4和CD8 T淋巴细胞及其亚群的百分比与冷冻保存的PBMC的结果。我们报告称，冷冻长达120天的WB淋巴细胞中的CD4和CD8值与冷冻长达10天的PBMC的CD4和CD8值非常相似。这些数据表明，在本研究调查的参数范围内，与我们最初的假设相反，保存在-80°C的全血实际上可能是免疫学和流行病学研究中T细胞计数检测的合适活淋巴细胞来源。
